Evidence map›Paper›PMID 29634774›Full record

ArticlePloS one2018

Dimeric IgG complexes from IVIg are incapable of inducing in vitro neutrophil degranulation or complement activation.

Iwan Kustiawan, Ninotska I L Derksen, Theresa Guhr, Simone Kruithof, Wim Jiskoot, Gestur Vidarsson, Theo Rispens

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Iwan KustiawanDepartment of Immunopathology, Sanquin Blood Supply and Landsteiner laboratory Amsterdam Medical Centre, University of Amsterdam, CX, Amsterdam, the Netherlands.
Ninotska I L DerksenDepartment of Immunopathology, Sanquin Blood Supply and Landsteiner laboratory Amsterdam Medical Centre, University of Amsterdam, CX, Amsterdam, the Netherlands.
Theresa GuhrDepartment of Immunopathology, Sanquin Blood Supply and Landsteiner laboratory Amsterdam Medical Centre, University of Amsterdam, CX, Amsterdam, the Netherlands.
Simone KruithofDepartment of Immunopathology, Sanquin Blood Supply and Landsteiner laboratory Amsterdam Medical Centre, University of Amsterdam, CX, Amsterdam, the Netherlands.
Wim JiskootDivision of Drug Delivery Technology, Cluster BioTherapeutics, Leiden Acedemic Centre of Drug Research (LACDR), Leiden, the Netherlands.
Gestur VidarssonDepartment of Immune heamatology, Sanquin Blood Supply and Landsteiner laboratory Amsterdam Medical Centre, University of Amsterdam, CX, Amsterdam, the Netherlands.ORCID 0000-0001-5621-003X
Theo RispensDepartment of Immunopathology, Sanquin Blood Supply and Landsteiner laboratory Amsterdam Medical Centre, University of Amsterdam, CX, Amsterdam, the Netherlands.
Sanquin · NLCentre for Human Drug Research · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeIntravenous immunoglobulin (IVIg) products contain various amounts of dimeric IgG complexes. Current insights into the possible biological activities of these dimers remain controversial, and both immunemodulating and immune-activating effects have been reported. Here, we analyzed the putative immune-activating effects of dimers isolated from IVIg.

methodsDimers isolated from IVIg were purified by high-performance size-exclusion chromatography (HP-SEC) and tested for the ability to induce neutrophil degranulation in vitro.

resultsDimers isolated from IVIg were found to be incapable of inducing in vitro neutrophil degranulation or complement activation, even at concentrations exceeding those expected to be reached upon administration in patients. These results depend on the removal of artefactual activation by using 0.1 micron filtration and the use of poloxamer to prevent adsorption of IgG onto the solid phase.

conclusionsThe data suggest dimeric IgG found in IVIg may bind to Fc-receptors without causing activation.

Indexed as

Cell DegranulationCells, CulturedComplement ActivationHumansImmunoglobulin GImmunoglobulins, IntravenousIn Vitro TechniquesNeutrophil ActivationNeutrophilsProtein MultimerizationImmunoglobulin GImmunoglobulins, Intravenous

Identifiers

PMID29634774
PMCPMC5892932
OpenAlexW2796873428

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.