Evidence map›Paper›PMID 29633913›Full record

ArticleJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2018

The use of pyrosequencing for detection of hemagglutinin mutations associated with increased pathogenicity of H5N1 avian influenza viruses in mammals.

Chenxi Wang, Yongning Zhang, Guoxia Bing, Xuxiao Zhang, Caixia Wang, Mingyang Wang, Yipeng Sun, Shaoqiang Wu, Xiangmei Lin, Juan Pu and 2 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Chenxi WangKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, China (Chenxi Wang, X Zhang, M Wang, Y Sun, Pu, Liu, H Sun).
Yongning ZhangKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, China (Chenxi Wang, X Zhang, M Wang, Y Sun, Pu, Liu, H Sun).
Guoxia BingKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, China (Chenxi Wang, X Zhang, M Wang, Y Sun, Pu, Liu, H Sun).
Xuxiao ZhangKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, China (Chenxi Wang, X Zhang, M Wang, Y Sun, Pu, Liu, H Sun).
Caixia WangKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, China (Chenxi Wang, X Zhang, M Wang, Y Sun, Pu, Liu, H Sun).
Mingyang WangKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, China (Chenxi Wang, X Zhang, M Wang, Y Sun, Pu, Liu, H Sun).
Yipeng SunKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, China (Chenxi Wang, X Zhang, M Wang, Y Sun, Pu, Liu, H Sun).
Shaoqiang WuKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, China (Chenxi Wang, X Zhang, M Wang, Y Sun, Pu, Liu, H Sun).
Xiangmei LinKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, China (Chenxi Wang, X Zhang, M Wang, Y Sun, Pu, Liu, H Sun).
Juan PuKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, China (Chenxi Wang, X Zhang, M Wang, Y Sun, Pu, Liu, H Sun).
Jinhua LiuKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, China (Chenxi Wang, X Zhang, M Wang, Y Sun, Pu, Liu, H Sun).
Honglei SunKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, China (Chenxi Wang, X Zhang, M Wang, Y Sun, Pu, Liu, H Sun).
China Agricultural University · CNChina Animal Disease Control Center · CNChinese Academy of Inspection and Quarantine · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hemagglutinin (HA) cleavage is critical for virulence of influenza viruses. The amino acid residue at the P6 position of the HA cleavage site (HACS) has been shown to be most variable and to have a direct correlation with the cleavage efficiency and pathogenicity of H5N1 avian influenza viruses (AIVs) in mammals. Among these amino acid variants, serine has been associated with the highest virulence in mammals, and its detection may serve as an indicator for H5N1 AIVs with high pathogenicity and potential public risk. We developed a rapid detection method based on reverse-transcription (RT)-PCR and pyrosequencing to detect a mutation at the HACS that is associated with increased pathogenicity of H5N1 AIVs in mammals. Herein, we provide a specific, sensitive, and reliable method for rapid detection of one of the virulence determinants associated with increased pathogenicity of H5N1 AIVs in mammals.

Indexed as

MammalsAnimalsHemagglutininsInfluenza A virusInfluenza A Virus, H5N1 SubtypeMutationOrthomyxoviridae InfectionsSelf-Sustained Sequence ReplicationVirulenceHemagglutininsH5N1hemagglutininpathogenicitypyrosequencingserine

Identifiers

PMID29633913
PMCPMC6505906
OpenAlexW2797595168

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.