ArticleEnvironmental science and pollution research international2018
Adenovirus and rotavirus recovery from a treated effluent through an optimized skimmed-milk flocculation method.
Article in Environmental science and pollution research international, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Global investigation of the presence of adenovirus in different types of water resources: a systematic review.Virusdisease · 2024Review
- Article
- Microbiological characterization of stormwater in a high-income neighborhood in Rio de Janeiro, Brazil.Environmental monitoring and assessment · 2022Article
- Global public health implications of human exposure to viral contaminated water.Frontiers in microbiology · 2022Review
- Evaluation of low-cost viral concentration methods in wastewaters: Implications for SARS-CoV-2 pandemic surveillances.Journal of virological methods · 2021Article
- Virological Characterization of Roof-Harvested Rainwater of Densely Urbanized Low-Income Region.Food and environmental virology · 2021Article
- Occurrence of various viruses and recent evidence of SARS-CoV-2 in wastewater systems.Journal of hazardous materials · 2021Review
- Detection and evaluation of rotavirus surveillance methods as viral indicator in the aquatic environments.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Review
- Rotavirus Surveillance in Tap Water, Recycled Water, and Sewage Sludge in Thailand: A Longitudinal Study, 2007-2018.Food and environmental virology · 2021Article
- Microbiological assessment of an urban lagoon system in the coastal zone of Rio de Janeiro, Brazil.Environmental science and pollution research international · 2021Article
- Viruses in wastewater: occurrence, abundance and detection methods.The Science of the total environment · 2020Review
- Molecular detection and genotyping of group A rotavirus in two wastewater treatment plants, Iran.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- The performance of biological and tertiary wastewater treatment procedures for rotaviruses A removal.Environmental science and pollution research international · 2020Article
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Authors and funding
5 authors.
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Abstract
Sewage treatment may be insufficient for the complete removal of enteric viruses, such as human adenoviruses (HAdV) and group A rotavirus (RVA). The differences in the efficiency of the treatment methodologies used may interfere with the detection of these viruses. The objective of this study was to optimize a skimmed-milk flocculation technique for the recovery of HAdV and RVA in the samples of treated effluent. The treated effluent collected at the wastewater treatment plant (WWTP) was processed via four protocols including modifications in the initial centrifugation step and the final concentration of skimmed-milk. The viral load and recovery rate were determined by quantitative PCR TaqMan® System. The highest recovery rates of HAdV, RVA, and bacteriophage PP7 (internal control process) were obtained when the concentration of skimmed-milk was doubled and no centrifugation step was used for the sample clarification. The optimized protocol was assessed in a field study conducted with 24 treated effluent samples collected bi-monthly during 2015. HAdV and RVA were detected in 50.0% (12/24) and 33.3% (08/24) of the samples tested, respectively, throughout the year, without seasonal variation (p > 0.05). This study corroborates the use of the organic flocculation method for virus recovery in environmental samples with the adaptation of the protocols to different aquatic matrices.
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