Evidence map›Paper›PMID 29632766›Full record

ArticlePlastic and reconstructive surgery. Global open2017

Quantification of Collagen Organization after Nerve Repair.

Jacqueline S Israel, Corinne R Esquibel, Aaron M Dingle, Yuming Liu, Adib Keikhosravi, Jane A Pisaniello, Madison A Hesse, Sarah K Brodnick, Joseph Novello, Lisa Krugner-Higby and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plastic and reconstructive surgery. Global open, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.5field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Advances in Imaging of Traumatic Nerve Injuries.The Journal of the American Academy of Orthopaedic Surgeons · 2025
    Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 1 institution in 1 country.

Jacqueline S IsraelDivision of Plastic Surgery, Department of Surgery, University of Wisconsin - Madison, Wis.; Laboratory for Optical and Computational Instrumentation, University of Wisconsin - Madison, Wis.; Department of Biomedical Engineering, College of Engineering, University of Wisconsin - Madison, Wis.; and Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin - Madison, Wis.
Corinne R EsquibelDivision of Plastic Surgery, Department of Surgery, University of Wisconsin - Madison, Wis.; Laboratory for Optical and Computational Instrumentation, University of Wisconsin - Madison, Wis.; Department of Biomedical Engineering, College of Engineering, University of Wisconsin - Madison, Wis.; and Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin - Madison, Wis.
Aaron M DingleDivision of Plastic Surgery, Department of Surgery, University of Wisconsin - Madison, Wis.; Laboratory for Optical and Computational Instrumentation, University of Wisconsin - Madison, Wis.; Department of Biomedical Engineering, College of Engineering, University of Wisconsin - Madison, Wis.; and Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin - Madison, Wis.
Yuming LiuDivision of Plastic Surgery, Department of Surgery, University of Wisconsin - Madison, Wis.; Laboratory for Optical and Computational Instrumentation, University of Wisconsin - Madison, Wis.; Department of Biomedical Engineering, College of Engineering, University of Wisconsin - Madison, Wis.; and Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin - Madison, Wis.
Adib KeikhosraviDivision of Plastic Surgery, Department of Surgery, University of Wisconsin - Madison, Wis.; Laboratory for Optical and Computational Instrumentation, University of Wisconsin - Madison, Wis.; Department of Biomedical Engineering, College of Engineering, University of Wisconsin - Madison, Wis.; and Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin - Madison, Wis.
Jane A PisanielloDivision of Plastic Surgery, Department of Surgery, University of Wisconsin - Madison, Wis.; Laboratory for Optical and Computational Instrumentation, University of Wisconsin - Madison, Wis.; Department of Biomedical Engineering, College of Engineering, University of Wisconsin - Madison, Wis.; and Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin - Madison, Wis.
Madison A HesseDivision of Plastic Surgery, Department of Surgery, University of Wisconsin - Madison, Wis.; Laboratory for Optical and Computational Instrumentation, University of Wisconsin - Madison, Wis.; Department of Biomedical Engineering, College of Engineering, University of Wisconsin - Madison, Wis.; and Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin - Madison, Wis.
Sarah K BrodnickDivision of Plastic Surgery, Department of Surgery, University of Wisconsin - Madison, Wis.; Laboratory for Optical and Computational Instrumentation, University of Wisconsin - Madison, Wis.; Department of Biomedical Engineering, College of Engineering, University of Wisconsin - Madison, Wis.; and Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin - Madison, Wis.
Joseph NovelloDivision of Plastic Surgery, Department of Surgery, University of Wisconsin - Madison, Wis.; Laboratory for Optical and Computational Instrumentation, University of Wisconsin - Madison, Wis.; Department of Biomedical Engineering, College of Engineering, University of Wisconsin - Madison, Wis.; and Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin - Madison, Wis.
Lisa Krugner-HigbyDivision of Plastic Surgery, Department of Surgery, University of Wisconsin - Madison, Wis.; Laboratory for Optical and Computational Instrumentation, University of Wisconsin - Madison, Wis.; Department of Biomedical Engineering, College of Engineering, University of Wisconsin - Madison, Wis.; and Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin - Madison, Wis.
Justin C WilliamsDivision of Plastic Surgery, Department of Surgery, University of Wisconsin - Madison, Wis.; Laboratory for Optical and Computational Instrumentation, University of Wisconsin - Madison, Wis.; Department of Biomedical Engineering, College of Engineering, University of Wisconsin - Madison, Wis.; and Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin - Madison, Wis.
Kevin W EliceiriDivision of Plastic Surgery, Department of Surgery, University of Wisconsin - Madison, Wis.; Laboratory for Optical and Computational Instrumentation, University of Wisconsin - Madison, Wis.; Department of Biomedical Engineering, College of Engineering, University of Wisconsin - Madison, Wis.; and Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin - Madison, Wis.
Samuel O PooreDivision of Plastic Surgery, Department of Surgery, University of Wisconsin - Madison, Wis.; Laboratory for Optical and Computational Instrumentation, University of Wisconsin - Madison, Wis.; Department of Biomedical Engineering, College of Engineering, University of Wisconsin - Madison, Wis.; and Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin - Madison, Wis.
University of Wisconsin–Madison · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundClinical outcomes after nerve injury and repair remain suboptimal. Patients may be plagued by poor functional recovery and painful neuroma at the repair site, characterized by disorganized collagen and sprouting axons. Collagen deposition during wound healing can be intrinsically imaged using second harmonic generation (SHG) microscopy. The purpose of this study was to develop a protocol for SHG imaging of nerves and to assess whether collagen alignment can be quantified after nerve repair.

methodsSciatic nerve transection and epineural repair was performed in male rats. The contralateral nerves were used as intra-animal controls. Ten-millimeter nerve segments were harvested and fixed onto slides. SHG images were collected using a 20× objective on a multiphoton microscope. Collagen fiber alignment was calculated using CurveAlign software. Alignment was calculated on a scale from 0 to 1, where 1 represents perfect alignment. Statistical analysis was performed using a linear mixed-effects model.

resultsEight male rats underwent right sciatic nerve repair using 9-0 Nylon suture. There were gross variations in collagen fiber organization in the repaired nerves compared with the controls. Quantitatively, collagen fibers were more aligned in the control nerves (mean alignment 0.754, SE 0.055) than in the repairs (mean alignment 0.413, SE 0.047;

conclusionsSHG microscopy can be used to quantitate collagen after nerve repair via fiber alignment. Given that the development of neuroma likely reflects aberrant wound healing, ex vivo and/or in vivo SHG imaging may be useful for further investigation of the variables predisposing to neuroma.

Identifiers

PMID29632766
PMCPMC5889458
OpenAlexW2772493851

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.