ArticleBiotechnology for biofuels2018
Flow cytometry analysis of
Article in Biotechnology for biofuels, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Bioconversion of C1-gases by mixotrophic co-cultures fermentation with C. carboxidivorans and C. beijerinkii.Bioresources and bioprocessing · 2025Article
- Lignocellulose-derived inhibitors can extend residence of Clostridium beijerinckii in active solventogenic state.Bioresources and bioprocessing · 2025Article
- Application of fed-batch strategy to fully eliminate the negative effect of lignocellulose-derived inhibitors in ABE fermentation.Biotechnology for biofuels and bioproducts · 2024Article
- Specificity of the AMP-6000 Method for EnumeratingFoods (Basel, Switzerland) · 2024Article
- Identification and Investigation of Autolysin Genes in Clostridium saccharoperbutylacetonicum Strain N1-4 for Enhanced Biobutanol Production.Applied and environmental microbiology · 2021Article
- Deeper below the surface-transcriptional changes in selected genes of Clostridium beijerinckii in response to butanol shock.MicrobiologyOpen · 2021Article
- Effect of aFrontiers in microbiology · 2021Article
- Changes in efflux pump activity of Clostridium beijerinckii throughout ABE fermentation.Applied microbiology and biotechnology · 2021Article
- Article
- Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives.Journal of industrial microbiology & biotechnology · 2020Review
- Phenotypic and Genomic Analysis ofFrontiers in bioengineering and biotechnology · 2020Article
- Metabolic Engineering of Histidine Kinases inFrontiers in bioengineering and biotechnology · 2020Article
- Matrices (re)loaded: Durability, viability, and fermentative capacity of yeast encapsulated in beads of different composition during long-term fed-batch culture.Biotechnology progress · 2020Article
- Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in Clostridium beijerinckii NRRL B-598 at the transcriptomic level.Scientific reports · 2019Article
- A transcriptional response ofBiotechnology for biofuels · 2019Article
- Quantification and isolation of Bacillus subtilis spores using cell sorting and automated gating.PloS one · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBiobutanol production by clostridia via the acetone-butanol-ethanol (ABE) pathway is a promising future technology in bioenergetics , but identifying key regulatory mechanisms for this pathway is essential in order to construct industrially relevant strains with high tolerance and productivity. We have applied flow cytometric analysis to
resultsDual staining by propidium iodide (PI) and carboxyfluorescein diacetate (CFDA) provided separation of cells into four subpopulations with different abilities to take up PI and cleave CFDA, reflecting different physiological states. The development of a staining pattern during ABE fermentation showed an apparent decline in viability, starting at the pH shift and onset of solventogenesis, although an appreciable proportion of cells continued to proliferate. This was observed for sporulating as well as non-sporulating phenotypes at low solvent concentrations, suggesting that the increase in percentage of inactive cells was not a result of solvent toxicity or a transition from vegetative to sporulating stages. Additionally, the sporulating phenotype was challenged with butanol and cultivation with a lower starting pH was performed; in both these experiments similar trends were obtained-viability declined after the pH breakpoint, independent of the actual butanol concentration in the medium. Production characteristics of both sporulating and non-sporulating phenotypes were comparable, showing that in
conclusionWe have shown that the decline in
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.