Evidence map›Paper›PMID 29632376›Full record

ArticleNature genetics2018

Highly parallel genome variant engineering with CRISPR-Cas9.

Meru J Sadhu, Joshua S Bloom, Laura Day, Jake J Siegel, Sriram Kosuri, Leonid Kruglyak

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
52citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

52 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. The power of many: when genetics met yeasts and high-throughput.Biological reviews of the Cambridge Philosophical Society · 2026
    Review
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Core gene set of the speciesbioRxiv : the preprint server for biology · 2025
    Article
  10. Article
  11. Comprehensive deletion scan of anti-CRISPR AcrIIA4 reveals essential and dispensable domains for Cas9 inhibition.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  12. Article
  13. Review
  14. Review
  15. Article
  16. Article
  17. Dissecting quantitative trait nucleotides by saturation genome editing.bioRxiv : the preprint server for biology · 2024
    Article
  18. Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Meru J Sadhu *Department of Human Genetics, University of California, Los Angeles, Los Angeles, CA, USA. msadhu@mednet.ucla.edu.ORCID http://orcid.org/0000-0002-8636-9102
Joshua S Bloom *Department of Human Genetics, University of California, Los Angeles, Los Angeles, CA, USA. jbloom@mednet.ucla.edu.
Laura DayDepartment of Human Genetics, University of California, Los Angeles, Los Angeles, CA, USA.
Jake J SiegelDepartment of Human Genetics, University of California, Los Angeles, Los Angeles, CA, USA.
Sriram KosuriInstitute for Quantitative and Computational Biology, University of California, Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0002-4661-0600
Leonid KruglyakDepartment of Human Genetics, University of California, Los Angeles, Los Angeles, CA, USA. lkruglyak@mednet.ucla.edu.ORCID http://orcid.org/0000-0002-8065-3057

Funding

Toward comprehensive genetic dissection of complex traits in yeastR01GM102308 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LEONID KRUGLYAK · 2012 to 2026
$3.3M
REVERSE GENOMICS OF REGULATORY ELEMENTS GOVERNING SPLICINGDP2GM114829 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KOSURI, SRIRAM · 2014 to 2014
$2.3M
Precise mapping of genetic variation using CRISPRF32GM116318 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI SADHU, MERU · 2016 to 2016
$60k
NIGMS NIH HHS DP2 GM114829NIGMS NIH HHS F32 GM116318NIGMS NIH HHS R01 GM102308
6 · The paper itself

Abstract

Understanding the functional effects of DNA sequence variants is of critical importance for studies of basic biology, evolution, and medical genetics; however, measuring these effects in a high-throughput manner is a major challenge. One promising avenue is precise editing with the CRISPR-Cas9 system, which allows for generation of DNA double-strand breaks (DSBs) at genomic sites matching the targeting sequence of a guide RNA (gRNA). Recent studies have used CRISPR libraries to generate many frameshift mutations genome wide through faulty repair of CRISPR-directed breaks by nonhomologous end joining (NHEJ)

Indexed as

CRISPR-Cas SystemsCodon, NonsenseDNA Breaks, Double-StrandedDNA, FungalGene EditingGenetic VariationGenome, FungalHumansModels, GeneticRNA, Guide, CRISPR-Cas SystemsSaccharomyces cerevisiaeCodon, NonsenseDNA, FungalRNA, Guide, CRISPR-Cas Systems

Identifiers

PMID29632376
PMCPMC6115196

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.