ArticleNature structural & molecular biology2018
Short cryptic exons mediate recursive splicing in Drosophila.
Article in Nature structural & molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Large-scale analysis of transcript data reveals thousands of recursive splicing events in human introns.bioRxiv : the preprint server for biology · 2026Article
- Detection of a sequence feature for recursive splicing.bioRxiv : the preprint server for biology · 2026Article
- Regulation of alternative splicing and polyadenylation in neurons.Life science alliance · 2023Review
- Comparative analysis of nascent RNA sequencing methods and their applications in studies of cotranscriptional splicing dynamics.The Plant cell · 2023Article
- Variations of intronic branchpoint motif: identification and functional implications in splicing and disease.Communications biology · 2023Review
- Exon definitive regions forMolecular therapy. Nucleic acids · 2023Article
- Transcription and splicing dynamics during earlyRNA (New York, N.Y.) · 2022Article
- Recursive splicing is a rare event in the mouse brain.PloS one · 2022Article
- Molecular and genetic dissection of recursive splicing.Life science alliance · 2022Article
- Differential fates of introns in gene expression due to global alternative splicing.Human genetics · 2022Review
- Article
- The Exon Junction Complex and intron removal prevent re-splicing of mRNA.PLoS genetics · 2021Article
- Antisense targeting of decoy exons can reduce intron retention and increase protein expression in human erythroblasts.RNA (New York, N.Y.) · 2020Article
- Exon Junction Complex Shapes the Transcriptome by Repressing Recursive Splicing.Molecular cell · 2018Article
- The temporal landscape of recursive splicing during Pol II transcription elongation in human cells.PLoS genetics · 2018Article
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3 authors.
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Abstract
Many long Drosophila introns are processed by an unusual recursive strategy. The presence of ~200 adjacent splice acceptor and splice donor sites, termed ratchet points (RPs), were inferred to reflect 'zero-nucleotide exons', whose sequential processing subdivides removal of long host introns. We used CRISPR-Cas9 to disrupt several intronic RPs in Drosophila melanogaster, some of which recapitulated characteristic loss-of-function phenotypes. Unexpectedly, selective disruption of RP splice donors revealed constitutive retention of unannotated short exons. Assays using functional minigenes confirm that unannotated cryptic splice donor sites are critical for recognition of intronic RPs, demonstrating that recursive splicing involves the recognition of cryptic RP exons. This appears to be a general mechanism, because canonical, conserved splice donors are specifically enriched in a 40-80-nt window downstream of known and newly annotated intronic RPs and exhibit similar properties to a broadly expanded class of expressed RP exons. Overall, these studies unify the mechanism of Drosophila recursive splicing with that in mammals.
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