ArticleScientific reports2018
Surface display of PbrR on Escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
- Innovative Approaches and Evolving Strategies in Heavy Metal Bioremediation: Current Limitations and Future Opportunities.Journal of xenobiotics · 2025Review
- Systematic evaluation of pigment-based whole-cell lead biosensors: challenges in genetic circuit engineering and critical considerations for background noise control.Frontiers in bioengineering and biotechnology · 2025Article
- Utilisation of acid-tolerant bacteria for base metal recovery under strongly acidic conditions.Extremophiles : life under extreme conditions · 2024Article
- Biosynthesis of Indigo Dyes and Their Application in Green Chemical and Visual Biosensing for Heavy Metals.ACS omega · 2024Article
- Synergistic vesicle-vector systems for targeted delivery.Journal of nanobiotechnology · 2024Review
- Ingestion of remediated lead-contaminated soils affects the fecal microbiome of mice.The Science of the total environment · 2022Article
- Potential Application of Living Microorganisms in the Detoxification of Heavy Metals.Foods (Basel, Switzerland) · 2022Review
- Detection of environmental pollutant cadmium in water using a visual bacterial biosensor.Scientific reports · 2022Article
- Article
- Differential Detection of Bioavailable Mercury and Cadmium Based on a Robust Dual-Sensing Bacterial Biosensor.Frontiers in microbiology · 2022Article
- Recent advances in bacterial biosensing and bioremediation of cadmium pollution: a mini-review.World journal of microbiology & biotechnology · 2021Review
- Indigoidine biosynthesis triggered by the heavy metal-responsive transcription regulator: a visual whole-cell biosensor.Applied microbiology and biotechnology · 2021Article
- Development of a bioavailable Hg(II) sensing system based on MerR-regulated visual pigment biosynthesis.Scientific reports · 2021Article
- Detection of Bioavailable Cadmium by Double-Color Fluorescence Based on a Dual-Sensing Bioreporter System.Frontiers in microbiology · 2021Article
- Development of Cadmium Multiple-Signal Biosensing and Bioadsorption Systems Based on ArtificialFrontiers in bioengineering and biotechnology · 2021Article
- Versatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury.PloS one · 2021Article
- Construction of a RFP-lacZα bicistronic reporter system and its application in lead biosensing.PloS one · 2020Article
- A SH3_5 Cell Anchoring Domain for Non-recombinant Surface Display on Lactic Acid Bacteria.Frontiers in bioengineering and biotechnology · 2020Article
- Improved Monitoring of Low-Level Transcription inFrontiers in microbiology · 2019Article
- Rapid monitoring of the target protein expression with a fluorescent signal based on a dicistronic construct in Escherichia coli.AMB Express · 2018Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human exposure to lead mainly occurs by ingestion of contaminated food, water and soil. Blocking lead uptake in the gastrointestinal tract is a novel prevention strategy. Whole-cell biosorbent for lead was constructed with PbrR genetically engineered on the cell surface of Escherichia coli (E. coli), a predominant strain among intestinal microflora, using lipoprotein (Lpp)-OmpA as the anchoring protein. In vitro, the PbrR displayed cells had an enhanced ability for immobilizing toxic lead(II) ions from the external media at both acidic and neutral pH, and exhibited a higher specific adsorption for lead compared to other physiological two valence metal ions. In vivo, the persistence of recombinant E. coli in the murine intestinal tract and the integrity of surface displayed PbrR were confirmed. In addition, oral administration of surface-engineered E. coli was safe in mice, in which the concentrations of physiological metal ions in blood were not affected. More importantly, lead associated with PbrR-displayed E. coli was demonstrated to be less bioavailable in the experimental mouse model with exposure to oral lead. This is reflected by significantly lower blood and femur lead concentrations in PbrR-displayed E. coli groups compared to the control. These results open up the possibility for the removal of toxic metal ions in vivo using engineered microorganisms as adsorbents.
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Registered trials
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