ReviewTissue engineering. Part B, Reviews2018
The Evolution of Polystyrene as a Cell Culture Material.
Review in Tissue engineering. Part B, Reviews, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
90 citing papers in PubMed, 254 citations in OpenAlex.
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- Electrospun 3D Nanofiber Scaffolds with Adhesion-Enhanced Interfaces for Cell Culture.ACS omega · 2026Article
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- Assessing the ability of bovine mesenchymal stem cells to grow on okara-based scaffolds derived from soy milk waste.Frontiers in nutrition · 2026Article
- Bioengineering Stem Cell-Derived Glioblastoma Organoids: A Comprehensive Review.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Antibiotic carry over is a confounding factor for cell-based antimicrobial research applications.Scientific reports · 2025Article
- Concentration-time profiles of the nerve agent VX and the pesticide parathion in five different cell culture plates.Archives of toxicology · 2025Article
- Endothelial dysfunction in cardiovascular diseases: mechanisms and in vitro models.Molecular and cellular biochemistry · 2025Review
- Nanofibrillar Cellulose Hydrogels with Anionic Surface Modifications for Modulating Macrophage Phenotype in 3D Culture.ACS applied materials & interfaces · 2025Article
- Review
- Repurposing Laboratory Plastic into Functional Fibrous Scaffolds via Green Electrospinning for Cell Culture and Tissue Engineering Applications.ACS biomaterials science & engineering · 2025Article
- Polystyrene substrate effects on human dental pulp cell behavior: implications for in vitro culture optimization.BMC oral health · 2025Article
- Engineering in vitro vascular microsystems.Microsystems & nanoengineering · 2025Review
- Engineered 3D-Printable Nanohydroxyapatite Biocomposites with Cold Plasma-Tailored Surface Features to Boost Osseointegration.ACS applied materials & interfaces · 2025Article
- Propagation of goose primordial germ cells in vitro relies on FGF and BMP signalling pathways.Communications biology · 2025Article
- An In Situ Characterisation Method for 3-D Electrospun Foams.Nanomaterials (Basel, Switzerland) · 2025Article
- Effects of Chemical Pretreatments of Wood Cellulose Nanofibrils on Protein Adsorption and Biological Outcomes.ACS applied materials & interfaces · 2025Article
30 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Polystyrene (PS) has brought in vitro cell culture from its humble beginnings to the modern era, propelling dozens of research fields along the way. This review discusses the development of the material, fabrication, and treatment approaches to create the culture material. However, native PS surfaces poorly facilitate cell adhesion and growth in vitro. To overcome this, liquid surface deposition, energetic plasma activation, and emerging functionalization methods transform the surface chemistry. This review seeks to highlight the many potential applications of the first widely accepted polymer growth surface. Although the majority of in vitro research occurs on two-dimensional surfaces, the importance of three-dimensional (3D) culture models cannot be overlooked. The methods to transition PS to specialized 3D culture surfaces are also reviewed. Specifically, casting, electrospinning, 3D printing, and microcarrier approaches to shift PS to a 3D culture surface are highlighted. The breadth of applications of the material makes it impossible to highlight every use, but the aim remains to demonstrate the versatility and potential as both a general and custom cell culture surface. The review concludes with emerging scaffolding approaches and, based on the findings, presents our insights on the future steps for PS as a tissue culture platform.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.