Evidence map›Paper›PMID 29631048›Full record

ArticleActa biomaterialia2018

Anisotropic biodegradable lipid coated particles for spatially dynamic protein presentation.

Randall A Meyer, Mohit P Mathew, Elana Ben-Akiva, Joel C Sunshine, Ron B Shmueli, Qiuyin Ren, Kevin J Yarema, Jordan J Green

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta biomaterialia, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Randall A MeyerDepartment of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA; Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA; Institute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD 21231, USA.
Mohit P MathewDepartment of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA; Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA.
Elana Ben-AkivaDepartment of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA; Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA.
Joel C SunshineDepartment of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA; Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA.
Ron B ShmueliDepartment of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA; Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA.
Qiuyin RenDepartment of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA; Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA.
Kevin J YaremaDepartment of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA; Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA.
Jordan J GreenDepartment of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA; Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA; Institute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD 21231, USA; Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21231, USA; Department of Ophthalmology, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA; Department of Oncology, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA; Department of Neurosurgery, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21231, USA. Electronic address: green@jhu.edu.

Funding

Wilmer Core Grant for Vision ResearchP30EY001765 · NEI · JOHNS HOPKINS UNIVERSITY · PI JEFFREY MUMM · 1985 to 2026
$22.1M
T32: Predoctoral and Postdoctoral Training Program in Nanotechnology for Cancer ResearchT32CA153952 · NCI · JOHNS HOPKINS UNIVERSITY · PI Denis Wirtz · 2015 to 2026
$3.5M
Gene Delivery Nanoparticles to Treat GlioblastomaR01EB016721 · NIBIB · JOHNS HOPKINS UNIVERSITY · PI GREEN, JORDAN · 2013 to 2017
$1.9M
Nanoparticle Modified Human Fat Derived Mesenchymal Stem Cells for Brain Cancer (Change of Organization Application)R01CA195503 · NCI · MAYO CLINIC JACKSONVILLE · PI GREEN, JORDAN, QUINONES-HINOJOSA, ALFREDO · 2016 to 2020
$1.9M
T-32 NIH Postdoctoral Training Program in Nanotechnology for Cancer Medicine NTCMT32CA130840 · NCI · JOHNS HOPKINS UNIVERSITY · PI WIRTZ, DENIS · 2008 to 2012
$1.2M
Biomimetic artificial antigen presenting cells for melanoma immunotherapyF31CA214147 · NCI · JOHNS HOPKINS UNIVERSITY · PI MEYER, RANDALL A · 2016 to 2017
$88k
NCI NIH HHS F31 CA214147NCI NIH HHS R01 CA195503NCI NIH HHS T32 CA130840NCI NIH HHS T32 CA153952NEI NIH HHS P30 EY001765NIBIB NIH HHS R01 EB016721
6 · The paper itself

Abstract

There has been growing interest in the use of particles coated with lipids for applications ranging from drug delivery, gene delivery, and diagnostic imaging to immunoengineering. To date, almost all particles with lipid coatings have been spherical despite emerging evidence that non-spherical shapes can provide important advantages including reduced non-specific elimination and increased target-specific binding. We combine control of core particle geometry with control of particle surface functionality by developing anisotropic, biodegradable ellipsoidal particles with lipid coatings. We demonstrate that these lipid coated ellipsoidal particles maintain advantageous properties of lipid polymer hybrid particles, such as the ability for modular protein conjugation to the particle surface using versatile bioorthogonal ligation reactions. In addition, they exhibit biomimetic membrane fluidity and demonstrate lateral diffusive properties characteristic of natural membrane proteins. These ellipsoidal particles simultaneously provide benefits of non-spherical particles in terms of stability and resistance to non-specific phagocytosis by macrophages as well as enhanced targeted binding. These biomaterials provide a novel and flexible platform for numerous biomedical applications. STATEMENT OF SIGNIFICANCE: The research reported here documents the ability of non-spherical polymeric particles to be coated with lipids to form anisotropic biomimetic particles. In addition, we demonstrate that these lipid-coated biodegradable polymeric particles can be conjugated to a wide variety of biological molecules in a "click-like" fashion. This is of interest due to the multiple types of cellular mimicry enabled by this biomaterial based technology. These features include mimicry of the highly anisotropic shape exhibited by cells, surface presentation of membrane bound protein mimetics, and lateral diffusivity of membrane bound substrates comparable to that of a plasma membrane. This platform is demonstrated to facilitate targeted cell binding while being resistant to non-specific cellular uptake. Such a platform could allow for investigations into how physical parameters of a particle and its surface affect the interface between biomaterials and cells, as well as provide biomimetic technology platforms for drug delivery and cellular engineering.

Indexed as

Coated Materials, BiocompatibleLipidsMembranes, ArtificialProteinsAnimalsAnisotropyMacrophagesMicePhagocytosisRAW 264.7 CellsCoated Materials, BiocompatibleLipidsMembranes, ArtificialProteinsBiomimeticLipidsMembrane fluidityParticle shapePolymers

Identifiers

PMID29631048
PMCPMC6084478

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.