ArticleACS nano2018
Differential Enzyme Flexibility Probed Using Solid-State Nanopores.
Article in ACS nano, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed.
- Solid-state nanopore sensing: the next workhorse of biophysical characterization.Nature methods · 2026Review
- Microfluidic Sample Compartmentalization for Biomolecular Concentration Quantification Using a Nanopore Sensor.Small methods · 2026Article
- Solid-state nanopore sensing reveals conformational changes induced by a mutation in a neuron-specific tRNAArg.Nucleic acids research · 2026Article
- Single-Molecule Techniques for Probing the Conformation, Dynamics and Interactions of Intrinsically Disordered Proteins.Biophysics reviews · 2025Article
- Large and Stable Nanopores Formed by Complement Component 9 for Characterizing Single Folded Proteins.ACS nano · 2025Article
- Localized Nanopore Fabrication in Silicon Nitride Membranes by Femtosecond Laser Exposure and Subsequent Controlled Breakdown.ACS applied materials & interfaces · 2025Article
- Angular-Inertia Regulated Stable and Nanoscale Sensing of Single Molecules Using Nanopore-In-A-Tube.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- In-tube micro-pyramidal silicon nanopore for inertial-kinetic sensing of single molecules.Nature communications · 2024Article
- Detection of Biomolecules Using Solid-State Nanopores Fabricated by Controlled Dielectric Breakdown.Sensors (Basel, Switzerland) · 2024Article
- Review
- Protein Sizing with 15 nm Conical Biological Nanopore YaxAB.ACS nano · 2023Article
- Wafer-Scale Fabrication of Uniform, Micrometer-Sized, Triangular Membranes on Sapphire for High-Speed Protein Sensing in a Nanopore.ACS applied materials & interfaces · 2023Article
- Investigation of α-Synuclein and Amyloid-β(42)-E22Δ Oligomers Using SiN Nanopore Functionalized with L-Dopa.Chemistry, an Asian journal · 2022Article
- PlyAB Nanopores Detect Single Amino Acid Differences in Folded Haemoglobin from Blood.Angewandte Chemie (International ed. in English) · 2022Article
- Localized Nanopore Fabrication via Controlled Breakdown.Nanomaterials (Basel, Switzerland) · 2022Review
- β-Barrel Nanopores with an Acidic-Aromatic Sensing Region Identify Proteinogenic Peptides at Low pH.ACS nano · 2022Article
- Article
- Real-Time and Label-Free Measurement of Deubiquitinase Activity with a MspA Nanopore.Chembiochem : a European journal of chemical biology · 2021Article
- The emerging landscape of single-molecule protein sequencing technologies.Nature methods · 2021Review
- Electrical unfolding of cytochromeProceedings of the National Academy of Sciences of the United States of America · 2021Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Enzymes and motor proteins are dynamic macromolecules that coexist in a number of conformations of similar energies. Protein function is usually accompanied by a change in structure and flexibility, often induced upon binding to ligands. However, while measuring protein flexibility changes between active and resting states is of therapeutic significance, it remains a challenge. Recently, our group has demonstrated that breadth of signal amplitudes in measured electrical signatures as an ensemble of individual protein molecules is driven through solid-state nanopores and correlates with protein conformational dynamics. Here, we extend our study to resolve subtle flexibility variation in dihydrofolate reductase mutants from unlabeled single molecules in solution. We first demonstrate using a canonical protein system, adenylate kinase, that both size and flexibility changes can be observed upon binding to a substrate that locks the protein in a closed conformation. Next, we investigate the influence of voltage bias and pore geometry on the measured electrical pulse statistics during protein transport. Finally, using the optimal experimental conditions, we systematically study a series of wild-type and mutant dihydrofolate reductase proteins, finding a good correlation between nanopore-measured protein conformational dynamics and equilibrium bulk fluorescence probe measurements. Our results unequivocally demonstrate that nanopore-based measurements reliably probe conformational diversity in native protein ensembles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.