ArticleJournal of visualized experiments : JoVE2018
Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 8 citations in OpenAlex.
- Stability of olfactory behavior syndromes in the Drosophila larva.Scientific reports · 2023Article
- Internal state affects local neuron function in an early sensory processing center to shape olfactory behavior in Drosophila larvae.Scientific reports · 2022Article
- Localization of muscarinic acetylcholine receptor-dependent rhythm-generating modules in theJournal of neurophysiology · 2022Article
- Ionotropic Receptor-dependent cool cells control the transition of temperature preference in Drosophila larvae.PLoS genetics · 2021Article
- Behavior Responses to Chemical and Optogenetic Stimuli inFrontiers in behavioral neuroscience · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The ability of insects to navigate toward odor sources is based on the activities of their first-order olfactory receptor neurons (ORNs). While a considerable amount of information has been generated regarding ORN responses to odorants, the role of specific ORNs in driving behavioral responses remains poorly understood. Complications in behavior analyses arise due to different volatilities of odorants that activate individual ORNs, multiple ORNs activated by single odorants, and the difficulty in replicating naturally observed temporal variations in olfactory stimuli using conventional odor-delivery methods in the laboratory. Here, we describe a protocol that analyzes Drosophila larval behavior in response to simultaneous optogenetic stimulation of its ORNs. The optogenetic technology used here allows for specificity of ORN activation and precise control of temporal patterns of ORN activation. Corresponding larval movement is tracked, digitally recorded, and analyzed using custom written software. By replacing odor stimuli with light stimuli, this method allows for a more precise control of individual ORN activation in order to study its impact on larval behavior. Our method could be further extended to study the impact of second-order projection neurons (PNs) as well as local neurons (LNs) on larval behavior. This method will thus enable a comprehensive dissection of olfactory circuit function and complement studies on how olfactory neuron activities translate in to behavior responses.
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Registered trials
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