ArticleJournal of visualized experiments : JoVE2018
Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- A novel stress response pathway mediates biofilm architecture in Pseudomonas aeruginosa.PLoS pathogens · 2026Article
- Understanding sub-cellular mechanochemical correlations of the neonatal extrahepatic bile duct extracellular matrix by atomic force microscope-Infrared spectroscopy.Analytica chimica acta · 2026Article
- Oxygen microenvironments inMicrobiology (Reading, England) · 2025Article
- Enhancing sweet sorghum emergence and stress resilience in saline-alkaline soils through ABA seed priming: insights into hormonal and metabolic reprogramming.BMC genomics · 2025Article
- The L-lactate dehydrogenases ofmBio · 2024Article
- The L-lactate dehydrogenases ofbioRxiv : the preprint server for biology · 2024Article
- Cellular arrangement impacts metabolic activity and antibiotic tolerance in Pseudomonas aeruginosa biofilms.PLoS biology · 2024Article
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- Spatial heterogeneity in biofilm metabolism elicited by local control of phenazine methylation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Changes in the Ultrastructure ofMicroorganisms · 2023Article
- Cell arrangement impacts metabolic activity and antibiotic tolerance inbioRxiv : the preprint server for biology · 2023Article
- A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration.Journal of visualized experiments : JoVE · 2023Article
- Effects of Exogenous Auxin on Mesocotyl Elongation of Sorghum.Plants (Basel, Switzerland) · 2023Article
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- Adaptation ofACS biomaterials science & engineering · 2021Article
- Pseudomonas aeruginosa PA14 produces R-bodies, extendable protein polymers with roles in host colonization and virulence.Nature communications · 2021Article
- Interdependency of Respiratory Metabolism and Phenazine-Associated Physiology in Pseudomonas aeruginosa PA14.Journal of bacteriology · 2020Article
- Phenazine production promotes antibiotic tolerance and metabolic heterogeneity in Pseudomonas aeruginosa biofilms.Nature communications · 2019Article
- Article
- The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning.Journal of visualized experiments : JoVE · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Sectioning via paraffin embedding is a broadly established technique in eukaryotic systems. Here we provide a method for the fixation, embedding, and sectioning of intact microbial colony biofilms using perfused paraffin wax. To adapt this method for use on colony biofilms, we developed techniques for maintaining each sample on its growth substrate and laminating it with an agar overlayer, and added lysine to the fixative solution. These optimizations improve sample retention and preservation of micromorphological features. Samples prepared in this manner are amenable to thin sectioning and imaging by light, fluorescence, and transmission electron microscopy. We have applied this technique to colony biofilms of Pseudomonas aeruginosa, Pseudomonas synxantha, Bacillus subtilis, and Vibrio cholerae. The high level of detail visible in samples generated by this method, combined with reporter strain engineering or the use of specific dyes, can provide exciting insights into the physiology and development of microbial communities.
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Registered trials
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