ArticleJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2018
Development and application of two multiplex real-time PCR assays for detection and speciation of bacterial pathogens in the koala.
Article in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Koala Conservation: The Utility of Art and Genome Banking.Advances in experimental medicine and biology · 2026Review
- The relationships of viral and protozoal co-infections to Chlamydia pecorum infection and chlamydiosis outcomes in northern koalas (Phascolarctos cinereus).PLoS pathogens · 2025Article
- Evidence of Folliculogenesis and the Potential of Oocyte Recovery from Koalas with Different Levels of Reproductive Pathology.Biology · 2025Article
- Evaluation of Chlamydia pecorum major outer membrane protein vaccine a management tool in wild koala (Phascolarctos cinereus) populations.Scientific reports · 2025Article
- Prognostic factors in bushfire-affected koalas-Kangaroo Island bushfire response 2020.Australian veterinary journal · 2025Article
- New insights into the range and transmission dynamics of a koala gammaherpesvirus, phascolarctid gammaherpesvirus 2.Scientific reports · 2025Article
- Advancements in noninvasive koala monitoring through combining Chlamydia detection with a targeted koala genotyping assay.Scientific reports · 2024Article
- Molecular survey for Chlamydia among southern greater gliders (Petauroides volans) from southeastern New South Wales, Australia.Veterinary research communications · 2024Article
- Molecular Detection and Characterisation ofPathogens (Basel, Switzerland) · 2024Article
- Immunohistochemical characterization of the immune cell response during chlamydial infection in the male and female koala (Veterinary pathology · 2024Article
- A novel multi-variate immunological approach, reveals immune variation associated with environmental conditions, and co-infection in the koala (Phascolarctos cinereus).Scientific reports · 2024Article
- Expanding the known distribution of phascolartid gammaherpesvirus 1 in koalas to populations across Queensland and New South Wales.Scientific reports · 2024Article
- Carriage of antibiotic resistance genes to treatments for chlamydial disease in koalas (One health (Amsterdam, Netherlands) · 2023Article
- The koala (Phascolarctos cinereus) prostate: a comprehensive histological and immunohistochemical investigation.Biology of reproduction · 2023Article
- Health Assessments of Koalas after Wildfire: A Temporal Comparison of Rehabilitated and Non-Rescued Resident Individuals.Animals : an open access journal from MDPI · 2023Article
- Article
- Development of diagnostic and point of care assays for a gammaherpesvirus infecting koalas.PloS one · 2023Article
- Helping koalas battle disease - Recent advances in Chlamydia and koala retrovirus (KoRV) disease understanding and treatment in koalas.FEMS microbiology reviews · 2020Review
- Rapid point-of-care diagnostics for the detection of Chlamydia pecorum in koalas (Phascolarctos cinereus) using loop-mediated isothermal amplification without nucleic acid purification.MicrobiologyOpen · 2019Article
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infectious diseases have contributed to the decline in the health of koala ( Phascolarctos cinereus) populations in the wild in some regions of Australia. Herein we report the development and validation of 2 multiplex real-time PCR (rtPCR) panels for the simultaneous detection of Mycoplasma spp., Ureaplasma spp., Bordetella bronchiseptica, and Chlamydia, including speciation and quantification of Chlamydia, in ocular, reproductive, and nasal swab samples in addition to semen and male urogenital and reproductive tissues, from koalas. Each rtPCR panel was developed for use as a single-tube reaction using pathogen-specific primers and fluorescently labeled probe sets. DNA extracted from reference strains and isolates was used for validation of sequence gene targets for the multiplex rtPCR panels. Each panel was shown to be sensitive and specific in detecting and differentiating the bacterial pathogens. The multiplex rtPCR panels were used to screen clinical samples from free-ranging and hospitalized koalas for multiple pathogens simultaneously. The multiplex rtPCR will improve turnaround time compared to individual-pathogen rtPCR methods used, to date, for confirmation of diagnosis and will provide the wildlife clinician with the ability to make treatment decisions more rapidly.
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