ArticleJournal of analytical methods in chemistry2018
A PCR Method That Can Be Further Developed into PCR-RFLP Assay for Eight Animal Species Identification.
Article in Journal of analytical methods in chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A plethora of laboratory protocols for vitamin D receptor (VDR) gene variants detection: a systematic review of associations with hypertensive disorders of pregnancy.BMC pregnancy and childbirth · 2025Pooled it
- Adaptation and Invasion Dynamics ofInsects · 2025Review
- Current State of Milk, Dairy Products, Meat and Meat Products, Eggs, Fish and Fishery Products Authentication and Chemometrics.Foods (Basel, Switzerland) · 2023Review
- Review
- Polymorphism analysis of myosin 1H (G/A) and P561T (C/A) genes on class I, class II, and class III malocclusion.Journal of orthodontic science · 2022Article
- Preliminary findings and molecular characterization of thin-walled Sarcocystis species in hearts of cattle and buffaloes in Thailand, Lao PDR, and Cambodia.Parasitology research · 2021Article
- Molecular identification of biwa trout (Journal of food science and technology · 2019Article
- Specific PCR method for detection of species origin in biochemical drugs via primers for the ATPase 8 gene by electrophoresis.Mikrochimica acta · 2019Article
- Sumateran wild boar (Veterinary world · 2019Article
- Mammalian species identification using ISSR-HRM technique.Science progressArticle
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There are many PCR-based methods for animal species identification; however, their detection numbers are limited or could not identify unknown species. We set out to solve this problem by developing a universal primer PCR assay for simultaneous identification of eight animal species, including goat, sheep, deer, buffalo, cattle, yak, pig, and camel. In this assay, the variable lengths of mitochondrial DNA were amplified using a pair of universal primers. PCR amplifications yielded 760 bp, 737 bp, 537 bp, 486 bp, 481 bp, 464 bp, 429 bp, and 359 bp length fragments for goat, sheep, deer, buffalo, cattle, yak, pig, and camel, respectively. This primer pair had no cross-reaction with other common domestic animals and fish. The limit of detection varied from 0.01 to 0.05 ng of genomic DNA for eight animal species in a 20
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.