ArticleChemical science2018
One-step methodology for the direct covalent capture of GPCRs from complex matrices onto solid surfaces based on the bioorthogonal reaction between haloalkane dehalogenase and chloroalkanes.
Article in Chemical science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 74 citations in OpenAlex.
- [Recent advances on bioaffinity fishing technology for discovery of active components from natural products].Se pu = Chinese journal of chromatography · 2026Review
- Pharmacodynamic material basis investigations of the crude and processed Radix et Rhizoma Rhei by the immobilized βMedicinal chemistry research : an international journal for rapid communications on design and mechanisms of action of biologically active agents · 2026Article
- A strategy for selective screening of dual-target bioactive compounds against hypertrophic scar through inhibiting angiotensin II type 1 receptor while stimulating type 2 receptor from Chinese herbs.Chinese medicine · 2025Article
- [Establishment and evaluation of haloalkane dehalogenase taggedSe pu = Chinese journal of chromatography · 2024Article
- Prediction of the binding interactions between rosmarinic acid and cysteinyl leukotriene receptor type 1 by molecular docking and immobilized receptor chromatography.RSC advances · 2024Article
- Immobilization of M3 Muscarinic Receptor to Rapidly Analyze Drug-Protein Interactions and Bioactive Components in a Natural Plant.International journal of molecular sciences · 2023Article
- Rapid One-Step Capturing of Native, Cell-Free Synthesized and Membrane-Embedded GLP-1R.International journal of molecular sciences · 2023Article
- Aptamer-assisted two-point immobilized agonist-bound angiotensin II type 1 receptor for a second-site modulator discovery.iScience · 2022Article
- Preparation and characterization of immobilized 5-HTJournal of pharmaceutical and biomedical analysis · 2022Article
- Tag-mediated single-step purification and immobilization of recombinant proteins toward protein-engineered advanced materials.Journal of advanced research · 2022Review
- Sensitive Oligodeoxynucleotide Synthesis Using Dim and Dmoc as Protecting Groups.The Journal of organic chemistry · 2019Article
- Screening Bioactive Compounds of Siraitia grosvenorii by Immobilized βFrontiers in pharmacology · 2019Article
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Corrections and comments
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Authors and funding
9 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein immobilization techniques play an important role in the development of assays for disease diagnosis and drug discovery. However, many of these approaches are not applicable to transmembrane proteins. G protein-coupled receptors (GPCRs) are the largest protein superfamily encoded by the human genome and are targeted by a quarter of all prescription drugs. GPCRs are highly dynamic and sensitive to changes in the ambient environment, and current immobilization methodologies are not suitable for GPCRs. We used haloalkane dehalogenase (Halo) as an immobilization tag fused to the β
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.