Evidence map›Paper›PMID 29628929›Full record

ArticleFrontiers in plant science2018

Multiplex PCR Targeted Amplicon Sequencing (MTA-Seq): Simple, Flexible, and Versatile SNP Genotyping by Highly Multiplexed PCR Amplicon Sequencing.

Yoshihiko Onda, Kotaro Takahagi, Minami Shimizu, Komaki Inoue, Keiichi Mochida

Open access · goldAbstract read
In one paragraph

Article in Frontiers in plant science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed
7.6field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 85 citations in OpenAlex.

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  19. Development of a genome-wide marker design workflow for onions and its application in target amplicon sequencing-based genotyping.DNA research : an international journal for rapid publication of reports on genes and genomes · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 1 country.

Yoshihiko OndaCellulose Production Research Team, RIKEN Center for Sustainable Resource Science, Yokohama, Japan.
Kotaro TakahagiCellulose Production Research Team, RIKEN Center for Sustainable Resource Science, Yokohama, Japan.
Minami ShimizuCellulose Production Research Team, RIKEN Center for Sustainable Resource Science, Yokohama, Japan.
Komaki InoueCellulose Production Research Team, RIKEN Center for Sustainable Resource Science, Yokohama, Japan.
Keiichi MochidaCellulose Production Research Team, RIKEN Center for Sustainable Resource Science, Yokohama, Japan.
RIKEN Center for Sustainable Resource Science · JPKihara Institute for Biological Research · JPOkayama University · JPYokohama City University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Next-generation sequencing (NGS) technologies have enabled genome re-sequencing for exploring genome-wide polymorphisms among individuals, as well as targeted re-sequencing for the rapid and simultaneous detection of polymorphisms in genes associated with various biological functions. Therefore, a simple and robust method for targeted re-sequencing should facilitate genotyping in a wide range of biological fields. In this study, we developed a simple, custom, targeted re-sequencing method, designated "multiplex PCR targeted amplicon sequencing (MTA-seq)," and applied it to the genotyping of the model grass

Indexed as

amplicon sequenceBrachypodium distachyongenotypingmarker panelnatural accessionSNP

Identifiers

PMID29628929
PMCPMC5876661
OpenAlexW2789847961

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.