Evidence map›Paper›PMID 29628395›Full record

ArticleStem cell reports2018

hPSC-Derived Striatal Cells Generated Using a Scalable 3D Hydrogel Promote Recovery in a Huntington Disease Mouse Model.

Maroof M Adil, Thomas Gaj, Antara T Rao, Rishikesh U Kulkarni, Christina M Fuentes, Gokul N Ramadoss, Freja K Ekman, Evan W Miller, David V Schaffer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Huntington's Disease: Complex Pathogenesis and Therapeutic Strategies.International journal of molecular sciences · 2024
    Review
  6. Review
  7. Article
  8. Article
  9. Review
  10. Cell reports methods · 2022
    Article
  11. Article
  12. Review
  13. Article
  14. Review
  15. Article
  16. Review
  17. Review
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Maroof M AdilDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA 94720, USA.
Thomas GajDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA.
Antara T RaoDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Rishikesh U KulkarniDepartment of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.
Christina M FuentesDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA.
Gokul N RamadossDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA 94720, USA.
Freja K EkmanDepartment of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.
Evan W MillerDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; The Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
David V SchafferDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; The Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA 94720, USA. Electronic address: schaffer@berkeley.edu.

Funding

Univ of Calif Berkeley Chemistry-Biology Interface ProgramT32GM066698 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI CHANG, CHRISTOPHER J., FRANCIS, MATTHEW B · 2004 to 2023
$7.5M
Stem Cell Engineering Training ProgramT32GM098218 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI HEALY, KEVIN EDWARD, SCHAFFER, DAVID V · 2011 to 2020
$1.8M
New Chemical Tools for Exploring Cellular PhysiologyR35GM119855 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI MILLER, EVAN WALKER · 2016 to 2020
$1.3M
Molecular and optogenetic tools for studying voltage in the brainR00NS078561 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI MILLER, EVAN WALKER · 2013 to 2015
$713k
Therapeutic genome editing for amyotrophic lateral sclerosisF32GM113446 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI GAJ, THOMAS · 2015 to 2017
$125k
NIGMS NIH HHS F32 GM113446NIGMS NIH HHS R35 GM119855NIGMS NIH HHS T32 GM066698NIGMS NIH HHS T32 GM098218NINDS NIH HHS R00 NS078561
6 · The paper itself

Abstract

Huntington disease (HD) is an inherited, progressive neurological disorder characterized by degenerating striatal medium spiny neurons (MSNs). One promising approach for treating HD is cell replacement therapy, where lost cells are replaced by MSN progenitors derived from human pluripotent stem cells (hPSCs). While there has been remarkable progress in generating hPSC-derived MSNs, current production methods rely on two-dimensional culture systems that can include poorly defined components, limit scalability, and yield differing preclinical results. To facilitate clinical translation, here, we generated striatal progenitors from hPSCs within a fully defined and scalable PNIPAAm-PEG three-dimensional (3D) hydrogel. Transplantation of 3D-derived striatal progenitors into a transgenic mouse model of HD slowed disease progression, improved motor coordination, and increased survival. In addition, the transplanted cells developed an MSN-like phenotype and formed synaptic connections with host cells. Our results illustrate the potential of scalable 3D biomaterials for generating striatal progenitors for HD cell therapy.

Indexed as

Action PotentialsAnimalsCell DifferentiationCell SurvivalCorpus StriatumDisease Models, AnimalHedgehog ProteinsHumansHuntington DiseaseHydrogelsMiceNeuronsPhenotypePluripotent Stem CellsWnt Signaling PathwayHedgehog ProteinsHydrogelsbiomaterialscell replacement therapydifferentiationhuman pluripotent stem cellsHuntington diseasemedium spiny neurons

Identifiers

PMID29628395
PMCPMC5995679

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.