ArticleCurrent genetics2018
Marker-free genetic manipulations in yeast using CRISPR/CAS9 system.
Article in Current genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Mitochondrially tethered Mmm1 can function as a sole lipid transporter at ER-mitochondria contacts.The Journal of cell biology · 2026Article
- EditingBiomolecules · 2026Review
- Exploring CRISPR/Cas9-Mediated Gene Editing Advances in Conventional and Non-conventional Yeast Species.Applied biochemistry and biotechnology · 2025Review
- A time-resolved framework for the recruitment of mRNP processing and assembly factors to a site of transcription.Nucleic acids research · 2025Article
- The GravyTrain toolbox for molecular cell biology.PloS one · 2025Article
- Rrm3 and Pif1 division of labor during replication through leading and lagging strand G-quadruplex.Nucleic acids research · 2024Article
- Protein fluorescent labeling in live yeast cells using scFv-based probes.Cell reports methods · 2022Article
- Transcription-replication coordination revealed in single live cells.Nucleic acids research · 2022Article
- Article
- Cac1 WHD and PIP domains have distinct roles in replisome progression and genomic stability.Current genetics · 2021Article
- A glance at genome editing with CRISPR-Cas9 technology.Current genetics · 2020Review
- Expanding the CRISPR/Cas9 Toolbox for Gene Engineering in S. cerevisiae.Current microbiology · 2020Article
- The gal80 Deletion by CRISPR-Cas9 in Engineered Saccharomyces cerevisiae Produces Artemisinic Acid Without Galactose Induction.Current microbiology · 2019Article
- CRISPR system in the yeast Saccharomyces cerevisiae and its application in the bioproduction of useful chemicals.World journal of microbiology & biotechnology · 2019Review
- Interactions in the ESCRT-III network of the yeast Saccharomyces cerevisiae.Current genetics · 2019Article
- Yeast genetic interaction screens in the age of CRISPR/Cas.Current genetics · 2019Review
- Pif1 is essential for efficient replisome progression through lagging strand G-quadruplex DNA secondary structures.Nucleic acids research · 2018Article
- Scarless genome editing: progress towards understanding genotype-phenotype relationships.Current genetics · 2018Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The budding yeast is currently one of the major model organisms for the study of a wide variety of biological processes. Genetic manipulation of yeast involves the extensive usage of selectable markers that can lead to undesired effects. Thus, marker-free genetic manipulation in yeast is highly desirable for gene/promoter replacement and various other applications. Here we combine the power of selectable markers followed by CRISPR/CAS9 genome editing for common genetic manipulations in yeast in a marker-free manner. We demonstrate our approach for whole gene and promoter replacements and for high-efficiency operator array integration. Our approach allows the utilization of many thousands of existing strains including library strains for the generation of significant genetic changes in yeast in a marker-free and cloning-free fashion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.