Evidence map›Paper›PMID 29625624›Full record

ArticleSkeletal muscle2018

An RNAi based screen in Drosophila larvae identifies fascin as a regulator of myoblast fusion and myotendinous junction structure.

Jaclyn M Camuglia, Torrey R Mandigo, Richard Moschella, Jenna Mark, Christine H Hudson, Derek Sheen, Eric S Folker

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Skeletal muscle, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Development and maintenance of tendons and ligaments.Development (Cambridge, England) · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Jaclyn M CamugliaBiology Department, Boston College, Chestnut Hill, MA, 02467, USA.
Torrey R MandigoBiology Department, Boston College, Chestnut Hill, MA, 02467, USA.
Richard MoschellaBiology Department, Boston College, Chestnut Hill, MA, 02467, USA.
Jenna MarkBiology Department, Boston College, Chestnut Hill, MA, 02467, USA.
Christine H HudsonBiology Department, Boston College, Chestnut Hill, MA, 02467, USA.
Derek SheenBiology Department, Boston College, Chestnut Hill, MA, 02467, USA.
Eric S FolkerBiology Department, Boston College, Chestnut Hill, MA, 02467, USA. eric.folker@bc.edu.ORCID 0000-0003-1619-7330
Boston College · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundA strength of Drosophila as a model system is its utility as a tool to screen for novel regulators of various functional and developmental processes. However, the utility of Drosophila as a screening tool is dependent on the speed and simplicity of the assay used.

methodsHere, we use larval locomotion as an assay to identify novel regulators of skeletal muscle function. We combined this assay with muscle-specific depletion of 82 genes to identify genes that impact muscle function by their expression in muscle cells. The data from the screen were supported with characterization of the muscle pattern in embryos and larvae that had disrupted expression of the strongest hit from the screen.

resultsWith this assay, we showed that 12/82 tested genes regulate muscle function. Intriguingly, the disruption of five genes caused an increase in muscle function, illustrating that mechanisms that reduce muscle function exist and that the larval locomotion assay is sufficiently quantitative to identify conditions that both increase and decrease muscle function. We extended the data from this screen and tested the mechanism by which the strongest hit, fascin, impacted muscle function. Compared to controls, animals in which fascin expression was disrupted with either a mutant allele or muscle-specific expression of RNAi had fewer muscles, smaller muscles, muscles with fewer nuclei, and muscles with disrupted myotendinous junctions. However, expression of RNAi against fascin only after the muscle had finished embryonic development did not recapitulate any of these phenotypes.

conclusionsThese data suggest that muscle function is reduced due to impaired myoblast fusion, muscle growth, and muscle attachment. Together, these data demonstrate the utility of Drosophila larval locomotion as an assay for the identification of novel regulators of muscle development and implicate fascin as necessary for embryonic muscle development.

Indexed as

RNA InterferenceAnimalsCarrier ProteinsCell FusionDrosophilaFemaleGene Expression RegulationLarvaMaleMicrofilament ProteinsMovementMuscle DevelopmentMuscle, SkeletalMyoblastsTendonsCarrier ProteinsfascinMicrofilament ProteinsDrosophilaFascinMyoblast fusionMyogenesisMyotendinous junctionNuclear movement

Identifiers

PMID29625624
PMCPMC5889537
OpenAlexW2799827862

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.