ArticleInternational journal for parasitology2018
Toxoplasma CRISPR/Cas9 constructs are functional for gene disruption in Neospora caninum.
Article in International journal for parasitology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Review
- Loss of NcBPK1 impairs bradyzoite differentiation and enhances virulence in Neospora caninum.Parasites & vectors · 2025Article
- Protective efficacy of the NcGRA7-deficient parasite as a live attenuated vaccine against Neospora caninum infection in mice.The Journal of veterinary medical science · 2025Article
- NcROP2 deletion reducesFrontiers in immunology · 2025Article
- Are genetically modified protozoa eligible for ATMP status? Concerning the legal categorization of an oncolytic protozoan drug candidate.Gene therapy · 2024Article
- TaqMan-quantitative PCR assays applied inFrontiers in cellular and infection microbiology · 2024Article
- NcPathogens (Basel, Switzerland) · 2022Article
- Article
- Disruption of Dense Granular Protein 2 (GRA2) Decreases the Virulence ofFrontiers in veterinary science · 2021Article
- Function of Neospora caninum dense granule protein 7 in innate immunity in mice.Parasitology research · 2021Article
- Effects of Dense Granular Protein 6 (GRA6) Disruption onFrontiers in veterinary science · 2020Article
- Neospora caninum Dense Granule Protein 7 Regulates the Pathogenesis of Neosporosis by Modulating Host Immune Response.Applied and environmental microbiology · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Herein we describe, to our knowledge for the first time the use of the clustered regularly interspaced short palindromic repeats/CRISPR-associated gene 9 (CRISPR/Cas9) system for genome editing of Neospora caninum, an apicomplexan parasite considered one of the main causes of abortion in cattle worldwide. By using plasmids containing the CRISPR/Cas9 components adapted to the closely related parasite Toxoplasma gondii, we successfully knocked out a green fluorescent protein (GFP) in an Nc-1 GFP-expressing strain, and efficiently disrupted the NcGRA7 gene in the Nc-Spain7 isolate by insertion of a pyrimethamine resistance cassette. The successful use of this technology in N. caninum lays the foundation for an efficient, targeted gene modification tool in this parasite.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.