ArticleMolecular biology reports2018
Effect of master mixes on the measurement of telomere length by qPCR.
Article in Molecular biology reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 13 citations in OpenAlex.
- Insights into the length and breadth of methodologies harnessed to study human telomeres.Biomarker research · 2024Article
- Genetic and clinical determinants of telomere length.HGG advances · 2023Article
- Comparing qPCR and DNA methylation-based measurements of telomere length in a high-risk pediatric cohort.Aging · 2022Article
- An Optimised Step-by-Step Protocol for Measuring Relative Telomere Length.Methods and protocols · 2020Article
- Uninterruptible Power Supply Improves Precision and External Validity of Telomere Length MeasurementExperimental results · 2020Article
- Telomere length measurement by qPCR - Summary of critical factors and recommendations for assay design.Psychoneuroendocrinology · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Alterations in telomere length (TL) have been associated with several diseases and a method based on qPCR, the Monochrome Multiplex Real-Time Quantitative PCR (MMQPCR) technique, has been used extensively for the analysis of TL. Some previous studies have been found that certain methodological conditions can affect the measurement of TL. The aim of the study was to evaluate the performance of eight different commercially available SYBR Green and High-Resolution Melting (HRM) mixes on the measurement of TL by the MMQPCR method. Four SYBR Green and four HRM mixes were tested and the measurement of TL was expressed by the T/S ratio. It was found that the type of master mix used in MMQPCR influences the measurement of TL, affecting aspects such as the specificity and consistency of the results. Our results are the first description of the effects of different master mixes on TL analysis by MMQPCR and highlight the importance of the future methodological improvement of this broadly used technique.
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Registered trials
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