Evidence map›Paper›PMID 29623285›Full record

ArticleBio-protocol2018

Quantification of Bacterial Attachment to Tissue Sections.

Batya Isaacson, Tehila Hadad, Gilad Bachrach, Ofer Mandelboim

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Adhesion ofVeterinary sciences · 2024
    Article
  2. Ex-Vivo Adhesion ofAnimals : an open access journal from MDPI · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Batya IsaacsonThe Lautenberg Center for General and Tumor Immunology, Department of Immunology and Cancer Research, IMRIC, Faculty of Medicine, the Hebrew University Medical School, Jerusalem, Israel.
Tehila HadadThe Institute of Dental Sciences, Hebrew University School of Dental Medicine, Jerusalem, Israel.
Gilad BachrachThe Institute of Dental Sciences, Hebrew University School of Dental Medicine, Jerusalem, Israel.
Ofer MandelboimThe Lautenberg Center for General and Tumor Immunology, Department of Immunology and Cancer Research, IMRIC, Faculty of Medicine, the Hebrew University Medical School, Jerusalem, Israel.

Funding

European Research Council 320473
6 · The paper itself

Abstract

Here we describe a method to test bacterial adhesion to paraffin embedded tissue sections. This method allows examining binding of different bacterial strains, transfected with a fluorescent protein reporter plasmid to various tissues, to better understand different mechanisms such as colonization. This assay provides a more physiological context to bacterial binding, than would have been achieved using adhesion assays to cell lines. The sections can be imaged using fluorescent microscopy and adhesion of various bacterial strains can be quantified and tested, simultaneously.

Indexed as

Bacterial attachmentBacterial colonizationHost-pathogen interactions

Identifiers

PMID29623285
PMCPMC5881888

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.