ReviewBeilstein journal of organic chemistry2018
Enzyme-free genetic copying of DNA and RNA sequences.
Review in Beilstein journal of organic chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Evolution of the substrate specificity of an RNA ligase ribozyme from phosphorimidazole to triphosphate activation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Template-based copying in chemically fuelled dynamic combinatorial libraries.Nature chemistry · 2024Article
- Template-Directed Replication and Chiral Resolution during Wet-Dry Cycling in Hydrothermal Pools.Life (Basel, Switzerland) · 2023Article
- Nonenzymatic Template-Directed Primer Extension Using 2'-3' Cyclic Nucleotides Under Wet-Dry Cycles.Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2023Article
- Growth, replication and division enable evolution of coacervate protocells.Chemical communications (Cambridge, England) · 2022Review
- Rolling Circles as a Means of Encoding Genes in the RNA World.Life (Basel, Switzerland) · 2022Article
- Thermodynamic and Kinetic Sequence Selection in Enzyme-Free Polymer Self-Assembly inside a Non-equilibrium RNA Reactor.Life (Basel, Switzerland) · 2022Article
- High Fidelity Enzyme-Free Primer Extension with an Ethynylpyridone Thymidine Analog.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Structure-Activity Relationships in Nonenzymatic Template-Directed RNA Synthesis.Angewandte Chemie (International ed. in English) · 2021Article
- Competition between bridged dinucleotides and activated mononucleotides determines the error frequency of nonenzymatic RNA primer extension.Nucleic acids research · 2021Article
- Review
- Potentially Prebiotic Activation Chemistry Compatible with Nonenzymatic RNA Copying.Journal of the American Chemical Society · 2020Article
- 2'/3' Regioselectivity of Enzyme-Free Copying of RNA Detected by NMR.Chembiochem : a European journal of chemical biology · 2020Article
- Deep sequencing of non-enzymatic RNA primer extension.Nucleic acids research · 2020Article
- Polyesters as a Model System for Building Primitive Biologies from Non-Biological Prebiotic Chemistry.Life (Basel, Switzerland) · 2020Article
- The difficult case of an RNA-only origin of life.Emerging topics in life sciences · 2019Article
- Continuous nonenzymatic cross-replication of DNA strands withChemical science · 2019Article
- Enzyme-free ligation of dimers and trimers to RNA primers.Nucleic acids research · 2019Article
- Template-Directed Catalysis of a Multistep Reaction Pathway for Nonenzymatic RNA Primer Extension.Biochemistry · 2019Article
- Incorporation of Sensitive Ester and Chloropurine Groups into Oligodeoxynucleotides through Solid Phase Synthesis.ChemistrySelect · 2018Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The copying of short DNA or RNA sequences in the absence of enzymes is a fascinating reaction that has been studied in the context of prebiotic chemistry. It involves the incorporation of nucleotides at the terminus of a primer and is directed by base pairing. The reaction occurs in aqueous medium and leads to phosphodiester formation after attack of a nucleophilic group of the primer. Two aspects of this reaction will be discussed in this review. One is the activation of the phosphate that drives what is otherwise an endergonic reaction. The other is the improved mechanistic understanding of enzyme-free primer extension that has led to a quantitative kinetic model predicting the yield of the reaction over the time course of an assay. For a successful modeling of the reaction, the strength of the template effect, the inhibitory effect of spent monomers, and the rate constants of the chemical steps have to be determined experimentally. While challenges remain for the high fidelity copying of long stretches of DNA or RNA, the available data suggest that enzyme-free primer extension is a more powerful reaction than previously thought.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.