ArticlePloS one2018
Fungal community profiles in agricultural soils of a long-term field trial under different tillage, fertilization and crop rotation conditions analyzed by high-throughput ITS-amplicon sequencing.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 109 citations in OpenAlex.
- Land-Use and Depth-Dependent Assembly of Soil Microbiomes Shapes Ecological Functions, Interaction Networks, and Phytopathogenic Communities Across Crop and Orchard Systems.Antioxidants (Basel, Switzerland) · 2026Article
- Predicting the absence of World Health Organization fungal priority pathogens in agricultural soils using machine learning and ITS metabarcoding.Die Naturwissenschaften · 2026Article
- Article
- Insights into fungal communities in some benchmark agricultural soils of Alberta, Canada.Microbiology (Reading, England) · 2026Article
- The molecular revolution in fungal diagnostics: bridging gaps across clinical, agricultural, and environmental mycology.Mycology · 2026Review
- Positive effects of reduced tillage intensity on soil organic matter are linked to shifts ofISME communications · 2026Article
- Soil cultivation methods rather than catch crop species enhance bacterial diversity in soil over a three-year experiment.Scientific reports · 2025Article
- Selective recruitment of beneficial microbes in the rhizosphere of maize affected by microbial inoculants, farming practice, and seasonal variations.Environmental microbiome · 2025Article
- Microbial inoculants modulate the rhizosphere microbiome, alleviate plant stress responses, and enhance maize growth at field scale.Genome biology · 2025Article
- The Fungal and Protist Community as Affected by Tillage, Crop Residue Burning and N Fertilizer Application.Current microbiology · 2025Article
- Long-term impact of tillage on microbial communities of an Eastern European Chernozem.Scientific reports · 2025Article
- Effects of strip-tillage on soil microbial community structure and function in black soil.Frontiers in microbiology · 2025Article
- Exploring Fungal Biodiversity in Crop Rotation Systems: Impact of Soil Fertility and Winter Wheat Cropping.Plants (Basel, Switzerland) · 2024Article
- Plant diversity drives positive microbial associations in the rhizosphere enhancing carbon use efficiency in agricultural soils.Nature communications · 2024Article
- Long-term conservation tillage with reduced nitrogen fertilization intensity can improve winter wheat health via positive plant-microorganism feedback in the rhizosphere.FEMS microbiology ecology · 2024Article
- Integrated management of fruit trees andFrontiers in microbiology · 2024Article
- Decline in Morel Production upon Continuous Cropping Is Related to Changes in Soil Mycobiome.Journal of fungi (Basel, Switzerland) · 2023Article
- Metabarcoding Reveals Impact of Different Land Uses on Fungal Diversity in the South-Eastern Region of Antioquia, Colombia.Plants (Basel, Switzerland) · 2023Article
- Testing plant growth promoting microorganisms in the field - a proposal for standards.Frontiers in plant science · 2023Review
- Green trees preservation: A sustainable source of valuable mushrooms for Ethiopian local communities.PloS one · 2023Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fungal communities in agricultural soils are assumed to be affected by soil and crop management. Our intention was to investigate the impact of different tillage and fertilization practices on fungal communities in a long-term crop rotation field trial established in 1992 in Central Germany. Two winter wheat fields in replicated strip-tillage design, comprising conventional vs. conservation tillage, intensive vs. extensive fertilization and different pre-crops (maize vs. rapeseed) were analyzed by a metabarcoding approach applying Illumina paired-end sequencing of amplicons generated by two recently developed primer pairs targeting the two fungal Internal Transcribed Spacer regions (ITS1, ITS2). Analysis of 5.1 million high-quality sequence reads uncovered a diverse fungal community in the two fields, composed of 296 fungal genera including 3,398 Operational Taxonomic Units (OTUs) at the 97% sequence similarity threshold. Both primer pairs detected the same fungal phyla (Basidio-, Asco-, Zygo-, Glomero- and Chytridiomycota), but in different relative abundances. OTU richness was higher in the ITS1 dataset, while ITS2 data were more diverse and of higher evenness. Effects of farming practice on fungal community structures were revealed. Almost two-thirds of the fungal genera were represented in all different soil treatments, whereas the remaining genera clearly responded to farming practice. Principal Component Analysis revealed four distinct clusters according to tillage practice and pre-crop. Analysis of Variance (ANOVA) substantiated the results and proved significant influences of tillage and pre-crop, while fertilization had the smallest and non-significant effect. In-depth analysis of putative phytopathogenic and plant beneficial fungal groups indicated distinct responses; for example Fusarium was significantly enriched in the intensively fertilized conservation tillage variants with the pre-crop maize, while Phoma displayed significant association with conventional tillage and pre-crop rapeseed. Many putative plant beneficial fungi also reacted differentially to farming practice with the most distinct responders identified among the Glomeromycota (arbuscular mycorrhizal fungi, AMF).
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