ArticleBiotechnology for biofuels2018
Engineering of
Article in Biotechnology for biofuels, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- D-CAPS: an efficient CRISPR-Cas9-based phage defense system forActa biochimica et biophysica Sinica · 2025Article
- Optimizing hexanoic acid biosynthesis in Saccharomyces cerevisiae for the de novo production of olivetolic acid.Biotechnology for biofuels and bioproducts · 2024Article
- Integration of (S)-2,3-oxidosqualene enables E. coli to become Iron Man E. coli with improved overall tolerance.Biotechnology for biofuels and bioproducts · 2023Article
- Flavour Volatiles of Fermented Vegetable and Fruit Substrates: A Review.Molecules (Basel, Switzerland) · 2023Review
- A kinetic framework for modeling oleochemical biosynthesis in Escherichia coli.Biotechnology and bioengineering · 2022Article
- Overview of the Cellular Stress Responses Involved in Fatty Acid Overproduction in E. coli.Molecular biotechnology · 2022Review
- Kinetically guided, ratiometric tuning of fatty acid biosynthesis.Metabolic engineering · 2022Review
- Wax ester production in nitrogen-rich conditions by metabolically engineeredMetabolic engineering communications · 2020Article
- The era of big data: Genome-scale modelling meets machine learning.Computational and structural biotechnology journal · 2020Review
- Development and Application of CRISPR/Cas in Microbial Biotechnology.Frontiers in bioengineering and biotechnology · 2020Review
- Engineering Escherichia coli FAB system using synthetic plant genes for the production of long chain fatty acids.Microbial cell factories · 2019Article
- Development of an autotrophic fermentation technique for the production of fatty acids using an engineered Ralstonia eutropha cell factory.Journal of industrial microbiology & biotechnology · 2019Article
- EngineeringPeerJ · 2019Article
- Biosynthesis, regulation, and engineering of microbially produced branched biofuels.Biotechnology for biofuels · 2019Review
- Lessons in Membrane Engineering for Octanoic Acid Production from Environmental Escherichia coli Isolates.Applied and environmental microbiology · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAs a versatile platform chemical, construction of microbial catalysts for free octanoic acid production from biorenewable feedstocks is a promising alternative to existing petroleum-based methods. However, the bio-production strategy has been restricted by the low capacity of
resultsFirst, a customized OptForce methodology was run to predict a set of four genetic interventions required for production of octanoic acid at 90% of the theoretical yield. Subsequently, all the ten candidate proteins associated with the predicted interventions were regulated individually, as well as in contrast to the combination of interventions as suggested by the OptForce strategy. Among these enzymes, increased production of 3-hydroxy-acyl-ACP dehydratase (FabZ) resulted in the highest increase (+ 45%) in octanoic acid titer. But importantly, the combinatorial application of FabZ with the other interventions as suggested by OptForce further improved octanoic acid production, resulting in a high octanoic acid-producing
conclusionsThis work demonstrates the effectiveness of integration of computational strain design and experimental characterization as a starting point in rewiring metabolism for octanoic acid production. This result in conjunction with the results of other studies using OptForce in strain design demonstrates that this strategy may be also applicable to engineering
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