Evidence map›Paper›PMID 29618668›Full record

ArticleThe Journal of veterinary medical science2018

Establishment of a Madin-Darby bovine kidney cell line expressing anchorless bovine prion protein.

Hyo-Jin Kim, In-Soon Roh, Hoo-Chang Park, Su Bi Ahn, Tae-Young Suh, Kyung-Je Park, Hae-Eun Kang, Hyun-Joo Sohn

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of veterinary medical science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Hyo-Jin KimOIE Reference Laboratory for CWD, Foreign Animal Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbukdo 39660, Korea.
In-Soon RohOIE Reference Laboratory for CWD, Foreign Animal Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbukdo 39660, Korea.
Hoo-Chang ParkOIE Reference Laboratory for CWD, Foreign Animal Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbukdo 39660, Korea.
Su Bi AhnOIE Reference Laboratory for CWD, Foreign Animal Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbukdo 39660, Korea.
Tae-Young SuhOIE Reference Laboratory for CWD, Foreign Animal Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbukdo 39660, Korea.
Kyung-Je ParkOIE Reference Laboratory for CWD, Foreign Animal Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbukdo 39660, Korea.
Hae-Eun KangOIE Reference Laboratory for CWD, Foreign Animal Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbukdo 39660, Korea.
Hyun-Joo SohnOIE Reference Laboratory for CWD, Foreign Animal Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbukdo 39660, Korea.
Animal and Plant Quarantine Agency · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Enzyme-linked immunosorbent assay (ELISA) performed using extensively purified bacterially expressed bovine prion protein (PrP) shows decreased cross-reactivity. We generated a transduced Madin-Darby bovine kidney (MDBK) cell line continuously expressing glycosylphosphatidylinositol (GPI)-anchorless bovine PrP (designated as MDBK ∆GPI protein) by using a lentiviral expression system. The present study also described the method for purifying bovine PrP through sequential culturing without the need for complex purification protocol. Our results showed that the purified bovine PrP could be used as an immunogen for developing anti-PrP monoclonal antibodies. Together, our results suggest that the new GPI-anchorless bovine PrP and its purification method can be used for performing basic studies for employing a cell-based approach.

Indexed as

AnimalsCattleCloning, MolecularDogsLentivirusMadin Darby Canine Kidney CellsPrion ProteinsTransduction, GeneticPrion ProteinsbovineGPI anchorlesslentivirusPrP

Identifiers

PMID29618668
PMCPMC6021889
OpenAlexW2796244857

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.