ArticleMicrobial cell factories2018
High-efficiency promoter-driven coordinated regulation of multiple metabolic nodes elevates lipid accumulation in the model microalga Phaeodactylum tricornutum.
Article in Microbial cell factories, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Stress-Induced Metabolic Reprogramming in the Green MicroalgaMicroorganisms · 2026Article
- Precision Microalgae: A New Conceptual Framework for Bioengineering Applications.Bioengineering (Basel, Switzerland) · 2026Review
- The marine diatom Phaeodactylum tricornutum as a versatile bioproduction chassis: Current progress, challenges, and perspectives.Plant communications · 2025Review
- Characterization of the VOC Promoter That Is Active Under Low-Salinity Conditions in the DiatomMarine drugs · 2025Article
- Comparative RNA-Seq of TenMarine drugs · 2024Article
- Isolation and Characterization of a Salt Inducible Promoter fromJournal of microbiology and biotechnology · 2023Article
- Advances in Genetic Engineering in Improving Photosynthesis and Microalgal Productivity.International journal of molecular sciences · 2023Review
- Unlocking microalgal host-exploring dark-growing microalgae transformation for sustainable high-value phytochemical production.Frontiers in bioengineering and biotechnology · 2023Article
- Emerging Trends in Genetic Engineering of Microalgae for Commercial Applications.Marine drugs · 2022Review
- Enhancing microalgal lipid accumulation for biofuel production.Frontiers in microbiology · 2022Review
- Highly Valuable Polyunsaturated Fatty Acids from Microalgae: Strategies to Improve Their Yields and Their Potential Exploitation in Aquaculture.Molecules (Basel, Switzerland) · 2021Review
- Method Development Progress in Genetic Engineering of Thraustochytrids.Marine drugs · 2021Review
- Advantages of Heterotrophic Microalgae as a Host for Phytochemicals Production.Frontiers in bioengineering and biotechnology · 2021Review
- Comparative Structural and Functional Analyses of the Fusiform, Oval, and Triradiate Morphotypes ofFrontiers in plant science · 2021Article
- A Review of Diatom Lipid Droplets.Biology · 2020Review
- Potential and Challenges of Improving Photosynthesis in Algae.Plants (Basel, Switzerland) · 2020Review
- Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application.Frontiers in bioengineering and biotechnology · 2020Review
- Identification and characterisation of the novel endogenous promoter HASP1 and its signal peptide from Phaeodactylum tricornutum.Scientific reports · 2019Article
- A novel endogenous selection marker for the diatom Phaeodactylum tricornutum based on a unique mutation in phytoene desaturase 1.Scientific reports · 2019Article
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Authors and funding
8 authors.
Funding
Abstract
backgroundMicroalgal metabolic engineering holds great promise for the overproduction of a wide range of commercial bioproducts. It demands simultaneous manipulation of multiple metabolic nodes. However, high-efficiency promoters have been lacking.
resultsHere we report a strong constitutive promoter Pt211 in expressing multiple target genes in oleaginous microalga Phaeodactylum tricornutum. Pt211 was revealed to contain significant cis-acting elements. GUS reporter and principal genes glycerol-3-phosphate acyltransferase (GPAT) and diacylglycerol acyltransferase 2 (DGAT2) involved in triacylglycerol biosynthesis were tested under driven of Pt211 in P. tricornutum. GUS staining and qPCR analysis showed strong GUS expression. DGAT2 and GPAT linked with a designed 2A sequence exhibited higher transcript abundances than WT, while algal growth and photosynthesis were not impaired.
conclusionThe total lipid content increased notably by 2.6-fold compared to WT and reached up to 57.5% (dry cell weight). Overall, our findings report a strong promoter and a strategy for coordinated manipulation of complex metabolic pathways.
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