ArticleEpigenetics & chromatin2018
Transient reduction of DNA methylation at the onset of meiosis in male mice.
Article in Epigenetics & chromatin, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 75 citations in OpenAlex.
- High-resolution chromatin mapping reveals that CTCF anchors meiotic loops to the chromosome axis.Nature communications · 2026Article
- Article
- The bull sperm epigenome: role in male fertility and transmission to the offspring.The Journal of reproduction and development · 2026Review
- Epigenetic regulation by DNA methylation, histone modifications and chromatin remodeling complexes in controlling spermatogenesis and their dysfunction with male infertility.Cellular and molecular life sciences : CMLS · 2025Review
- DNA methylation at retrotransposons protects the germline by preventing NRF1-mediated activation.EMBO reports · 2025Article
- Analysis of DNA methylation changes following Cfp1 knockout in mouse spermatocytes.Animal bioscience · 2025Article
- Mechanisms and reversibility of nicotine-induced spermatogenesis impairment and DNA methylation changes.Communications biology · 2025Article
- SETD1B-mediated broad H3K4me3 controls proper temporal patterns of gene expression critical for spermatid development.Cell research · 2025Article
- Intercellular bridges are essential for transposon repression and meiosis in the male germline.Nature communications · 2025Article
- Site-specific DNA demethylation during spermatogenesis presets the sites of nucleosome retention in mouse sperm.bioRxiv : the preprint server for biology · 2025Article
- Heterochromatin in plant meiosis.Nucleus (Austin, Tex.) · 2024Review
- Article
- DNA cytosine methyltransferases differentially regulate genome-wide hypermutation and interhomolog recombination in Trichoderma reesei meiosis.Nucleic acids research · 2024Article
- Genome-wide DNA methylation changes in human spermatogenesis.American journal of human genetics · 2024Article
- High resolution maps of chromatin reorganization through mouse meiosis reveal novel features of the 3D meiotic structure.bioRxiv : the preprint server for biology · 2024Article
- Single-cell multi-omics sequencing of human spermatogenesis reveals a DNA demethylation event associated with male meiotic recombination.Nature cell biology · 2023Article
- Retrotransposon LINE-1 bodies in the cytoplasm of piRNA-deficient mouse spermatocytes: Ribonucleoproteins overcoming the integrated stress response.PLoS genetics · 2023Article
- Ectopic expression of meiotic cohesin generates chromosome instability in cancer cell line.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Epigenetic responses to heat: From adaptation to maladaptation.Experimental physiology · 2022Review
- Prepubertal nutritional modulation in the bull and its impact on sperm DNA methylation.Cell and tissue research · 2022Article
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundMeiosis is a specialized germ cell cycle that generates haploid gametes. In the initial stage of meiosis, meiotic prophase I (MPI), homologous chromosomes pair and recombine. Extensive changes in chromatin in MPI raise an important question concerning the contribution of epigenetic mechanisms such as DNA methylation to meiosis. Interestingly, previous studies concluded that in male mice, genome-wide DNA methylation patters are set in place prior to meiosis and remain constant subsequently. However, no prior studies examined DNA methylation during MPI in a systematic manner necessitating its further investigation.
resultsIn this study, we used genome-wide bisulfite sequencing to determine DNA methylation of adult mouse spermatocytes at all MPI substages, spermatogonia and haploid sperm. This analysis uncovered transient reduction of DNA methylation (TRDM) of spermatocyte genomes. The genome-wide scope of TRDM, its onset in the meiotic S phase and presence of hemimethylated DNA in MPI are all consistent with a DNA replication-dependent DNA demethylation. Following DNA replication, spermatocytes regain DNA methylation gradually but unevenly, suggesting that key MPI events occur in the context of hemimethylated genome. TRDM also uncovers the prior deficit of DNA methylation of LINE-1 retrotransposons in spermatogonia resulting in their full demethylation during TRDM and likely contributing to the observed mRNA and protein expression of some LINE-1 elements in early MPI.
conclusionsOur results suggest that contrary to the prevailing view, chromosomes exhibit dynamic changes in DNA methylation in MPI. We propose that TRDM facilitates meiotic prophase processes and gamete quality control.
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