ArticlePoultry science2018
Development and application of a SYBR green real-time PCR for detection of the emerging avian leukosis virus subgroup K.
Article in Poultry science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- A novel recombinant ALV-K carrying ALV-J-derived LTR, pol, and 3'UTR from Chinese indigenous chicken breeds exhibits enhanced replication and increased hemangioma-associated pathogenicity.BMC veterinary research · 2026Article
- Generation of chimeric chickens resistant to infection by avian leukemia virus subgroups A, B, D, E, and K through primordial germ cells.Poultry science · 2026Article
- Cascaded signal amplification strategy for ultra-specific, ultra-sensitive, and visual detection of Shigella flexneri.Mikrochimica acta · 2024Article
- Article
- Chicken telomerase reverse transcriptase promotes the tumorigenicity of avian leukosis virus subgroup J by regulating the Wnt/β-catenin signaling pathway.Veterinary research · 2022Article
- Label-free fluorescent aptasensor for chloramphenicol based on hybridization chain reaction amplification and G-quadruplex/RSC advances · 2022Article
- New rapid detection by using a constant temperature method for avian leukosis viruses.Frontiers in microbiology · 2022Article
- Development of an SYBR Green I-based real-time PCR assay for the rapid detection of canine kobuvirus.Journal of virological methods · 2020Article
- Newly detected mutations in the Meq oncogene and molecular pathotyping of very virulent Marek's disease herpesvirus in Tunisia.Archives of virology · 2020Article
- A sensitive fluorometric sensor for AgRSC advances · 2020Article
- A Novel, Rapid, and Simple PMA-qPCR Method for Detection and Counting of ViableJournal of veterinary research · 2020Article
- Rapid detection of avian leukosis virus using a fluorescent microsphere immunochromatographic test strip assay.Poultry science · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Avian leukosis virus subgroup K (ALV-K) is an emerging ALV tumor virus of chickens. We developed a SYBR green-based real-time polymerase chain reaction (PCR) assay for the rapid and economical detection of ALV-K in chicken flocks. The assay was specific for ALV-K and did not cross-react with other ALV subgroup or avian influenza virus, Newcastle disease virus, or Marek's Disease virus. The method was 100 times more sensitive than conventional PCR and 10 times more sensitive than the enzyme-linked immunosorbent assay (ELISA) for the P27 antigen. The assay was also more sensitive than conventional PCR in tests of 86 clinical plasma samples. DF-1 tissue culture cells infected with 1 TCID50 ALV-K particle were identified as negative using ELISA but tested positive with the real-time PCR method. The viral loads in organs and tissues in infected chickens were highest in kidney, lungs, and glandular stomach, and these results matched ELISA findings.
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