ArticleThe Journal of cell biology1987
A fibronectin matrix is required for differentiation of murine erythroleukemia cells into reticulocytes.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 162 citations in OpenAlex.
- The role of ORC4 in enucleation of Murine Erythroleukemia (MEL) cells is similar to that in oocyte polar body extrusion.Systems biology in reproductive medicine · 2020Article
- Reappraising the role of α5 integrin and the microenvironmental support in stress erythropoiesis.Experimental hematology · 2020Article
- Article
- The Asymmetric Cell Division Regulators Par3, Scribble and Pins/Gpsm2 Are Not Essential for Erythroid Development or Enucleation.PloS one · 2017Article
- Article
- The sorting of blood group active proteins during enucleation.ISBT science series · 2015Article
- Mouse fetal liver culture system to dissect target gene functions at the early and late stages of terminal erythropoiesis.Journal of visualized experiments : JoVE · 2014Article
- Article
- Modulation of Tumor Cell Survival, Proliferation, and Differentiation by the Peptide Derived from Tenascin-C: Implication of β1-Integrin Activation.International journal of cell biology · 2012Article
- Formation of mammalian erythrocytes: chromatin condensation and enucleation.Trends in cell biology · 2011Review
- Embryonic regulation of the mouse hematopoietic niche.TheScientificWorldJournal · 2011Review
- Erythroblast enucleation.Stem cells international · 2011Article
- Histone deacetylase 2 is required for chromatin condensation and subsequent enucleation of cultured mouse fetal erythroblasts.Haematologica · 2010Article
- A transcriptome-based examination of blood group expression.Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine · 2010Article
- Article
- VLA-5-mediated adhesion to fibronectin accelerates hemin-stimulated erythroid differentiation of K562 cells through induction of VLA-4 expression.The Journal of biological chemistry · 2009Article
- Dynamics of human erythroblast enucleation.International journal of hematology · 2008Article
- Review
- Role of Gas6 in erythropoiesis and anemia in mice.The Journal of clinical investigation · 2008Article
- Alpha4beta1 integrin and erythropoietin mediate temporally distinct steps in erythropoiesis: integrins in red cell development.The Journal of cell biology · 2007Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Erythroid differentiation of murine erythroleukemia (MEL) cells is far more extensive when the cells are attached to fibronectin-coated dishes than in suspension culture. Cells induced in suspension culture for 4 d become arrested at a late erythroblast stage and do not undergo enucleation. Incubation of cells in suspension beyond 4 d results in lysis. In contrast, cells induced by DMSO on fibronectin-coated dishes for 7 d differentiate into enucleating cells, reticulocytes, and erythrocytes. As determined by quantitative immunoblotting, cells induced in suspension culture accumulate approximately 33% of the amount of the major erythroid membrane protein Band 3 present in erythrocyte, whereas cells induced on fibronectin-coated dishes accumulate 80-100% of the amount present in erythrocytes. Both suspension-induced cells and cells induced on fibronectin-coated dishes accumulate approximately 90% of the amount of spectrin and ankyrin present in erythrocytes. As revealed by immunofluorescence microscopy during enucleation of MEL cells, both Band 3 and ankyrin are sequestered in the cytoplasmic fragment of the emerging reticulocyte. Enucleated and later-stage cells detach from the fibronectin matrix, due to the loss of the surface fibronectin receptor; this mimics the normal release of reticulocytes from the matrix of the bone marrow into the blood. Thus a fibronectin matrix provides a permissive microenvironment within which erythroid precursor cells reside, proliferate, migrate, and express their normal differentiation program.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.