ArticleScientific reports2018
Centrifugation-free extraction of circulating nucleic acids using immiscible liquid under vacuum pressure.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Guidelines for pre-analytical conditions for assessing the methylation of circulating cell-free DNA.Clinical epigenetics · 2021Pooled it
- Proof of concept for aqueous two-phase system-based extraction of cell-free DNA from plasma for liquid biopsy applications.Scientific reports · 2026Article
- Improving electrochemical hybridization assays with restriction enzymes.Chemical communications (Cambridge, England) · 2024Article
- The impact of preanalytical variables on the analysis of cell-free DNA from blood and urine samples.Frontiers in cell and developmental biology · 2024Review
- Development of RNA/DNA automated extraction and purification device for infectious disease diagnosis.Practical laboratory medicine · 2023Article
- Diagnostic performance of respirators for collection and detection of SARS-CoV-2.Scientific reports · 2023Article
- A Review of Detection Methods for Vancomycin-ResistantBiosensors · 2023Review
- Rapid and Efficient Extraction of Cell-Free DNA Using Homobifunctional Crosslinkers.Biomedicines · 2022Article
- Improved DNA extraction on bamboo paper and cotton is tightly correlated with their crystallinity and hygroscopicity.PloS one · 2022Article
- A low-cost, open-source centrifuge adaptor for separating large volume clinical blood samples.PloS one · 2022Article
- Analytical Technologies for Liquid Biopsy of Subcellular Materials.Annual review of analytical chemistry (Palo Alto, Calif.) · 2021Article
- Technical and Methodological Aspects of Cell-Free Nucleic Acids Analyzes.International journal of molecular sciences · 2020Review
- Review
- The Different Facets of Liquid Biopsy: A Kaleidoscopic View.Cold Spring Harbor perspectives in medicine · 2020Review
- Rapid and Efficient Isolation of Exosomes by Clustering and Scattering.Journal of clinical medicine · 2020Article
- Solid-Phase Microextraction Enables Isolation of BRAF V600E Circulating Tumor DNA from Human Plasma for Detection with a Molecular Beacon Loop-Mediated Isothermal Amplification Assay.Analytical chemistry · 2020Article
- Precision cell-free DNA extraction for liquid biopsy by integrated microfluidics.NPJ precision oncology · 2020Article
- Simple and Low-Cost Sampling of Cell-Free Nucleic Acids from Blood Plasma for Rapid and Sensitive Detection of Circulating Tumor DNA.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Circulating DNA, a Potentially Sensitive and Specific Diagnostic Tool for Future Medicine.Dose-response : a publication of International Hormesis SocietyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extraction of cell-free DNA (cfDNA), which exists at an extremely low concentration in plasma, is a critical process for either targeted-sensing or massive sequencing of DNAs. However, such small amount of DNA cannot be fully obtained without high-speed centrifugation (<20,000 g). Here, we developed a centrifugation-free cfDNA extraction method and system that utilizes an immiscible solvent under single low vacuum pressure throughout the entire process. It has been named Pressure and Immiscibility-Based EXtraction (PIBEX). The amounts of extracted cfDNA by PIBEX were compared with those extracted by the conventional gold standards such as QIAGEN using quantitative PCR (qPCR). The PIBEX system showed equal performance regarding extraction amount and efficiency compared to the existing method. Because the PIBEX eliminates the troublous and repetitive centrifugation processes in DNA extraction, it can be further utilized in microfluidic-sample preparation systems for circulating nucleic acids, which would lead to an integrated sample-to-answer system in liquid biopsies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.