ArticleScientific reports2018
Targeted expression of step-function opsins in transgenic rats for optogenetic studies.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 14 citations in OpenAlex.
- Transcranial Calcium Macro-Imaging From the Auditory Cortex of Thy1-Cre-Driven GCaMP8 Transgenic Rats.Neuropsychopharmacology reports · 2026Article
- Intrinsic responses to hypoxia and hypercapnia of neurons in the cardiorespiratory center of the ventral medulla of newborn rats.Pflugers Archiv : European journal of physiology · 2025Article
- Advances in optogenetic studies of depressive-like behaviors and underlying neural circuit mechanisms.Frontiers in psychiatry · 2022Review
- Application of Optogenetics for Muscle Cells and Stem Cells.Advances in experimental medicine and biology · 2021Article
- Optogenetic Approach to Local Neuron Network Analysis of the Medullary Respiratory Center.Advances in experimental medicine and biology · 2021Article
- Driving Neurogenesis in Neural Stem Cells with High Sensitivity Optogenetics.Neuromolecular medicine · 2020Article
- New era of optogenetics: from the central to peripheral nervous system.Critical reviews in biochemistry and molecular biology · 2020Review
- Future Portrait of the Athletic Brain: Mechanistic Understanding of Human Sport PerformanceFrontiers in systems neuroscience · 2020Article
- Optogenetic analysis of respiratory neuronal networks in the ventral medulla of neonatal rats producing channelrhodopsin in Phox2b-positive cells.Pflugers Archiv : European journal of physiology · 2019Article
- Ipsilateral-Dominant Control of Limb Movements in Rodent Posterior Parietal Cortex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
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Authors and funding
15 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rats are excellent animal models for experimental neuroscience. However, the application of optogenetics in rats has been hindered because of the limited number of established transgenic rat strains. To accomplish cell-type specific targeting of an optimized optogenetic molecular tool, we generated ROSA26/CAG-floxed STOP-ChRFR(C167A)-Venus BAC rats that conditionally express the step-function mutant channelrhodopsin ChRFR(C167A) under the control of extrinsic Cre recombinase. In primary cultured cortical neurons derived from this reporter rat, only Cre-positive cells expressing ChRFR(C167A) became bi-stable, that is, their excitability was enhanced by blue light and returned to the baseline by yellow~red light. In bigenic pups carrying the Phox2B-Cre driver, ChRFR(C167A) was specifically expressed in the rostral parafacial respiratory group (pFRG) in the medulla, where endogenous Phox2b immunoreactivity was detected. These neurons were sensitive to blue light with an increase in the firing frequency. Thus, this transgenic rat actuator/reporter system should facilitate optogenetic studies involving the effective in vivo manipulation of the activities of specific cell fractions using light of minimal intensity.
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