ArticleScientific reports2018
Unbiased Strain-Typing of Arbovirus Directly from Mosquitoes Using Nanopore Sequencing: A Field-forward Biosurveillance Protocol.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 53 citations in OpenAlex.
- Exploration of viral diversity in Aedes mosquitoes employing different shotgun metagenomic data analysis pipelines.Virology journal · 2026Article
- Metagenomic profiling unveils the viral diversity in field-collected Aedes larvae from Central India employing nanopore sequencing.Scientific reports · 2026Article
- Going Mobile: Using Portable Genomic Technologies for PCR-Free In Situ Species Identification and Real-Time Molecular Systematics.Ecology and evolution · 2025Article
- Variability and bias in microbiome metagenomic sequencing: an interlaboratory study comparing experimental protocols.Scientific reports · 2024Article
- Influence of dengue virus serotypes on the abundance ofJournal of virology · 2024Article
- Technical comparison of MinIon and Illumina technologies for genotyping Chikungunya virus in clinical samples.Journal, genetic engineering & biotechnology · 2023Article
- Metagenomic surveillance for bacterial tick-borne pathogens using nanopore adaptive sampling.Scientific reports · 2023Article
- Unbiased Virus Detection in a Danish Zoo Using a Portable Metagenomic Sequencing System.Viruses · 2023Article
- Review
- Impact of nanopore-based metagenome sequencing on tick-borne virus detection.Frontiers in microbiology · 2023Article
- Rapid genotyping of porcine reproductive and respiratory syndrome virus (PRRSV) using MinION nanopore sequencing.PloS one · 2023Article
- Improvements in metagenomic virus detection by simple pretreatment methods.Journal of clinical virology plus · 2022Article
- Metagenomics to Detect and Characterize Viruses in Food Samples at Genome Level? Lessons Learnt from a Norovirus Study.Foods (Basel, Switzerland) · 2022Article
- Rapid, in-field deployable, avian influenza virus haemagglutinin characterisation tool using MinION technology.Scientific reports · 2022Article
- NOMAD: metagenomic characterisation of the viral pathogen composition in outbreaks of non-malaria acute febrile illness cases.Open research Africa · 2022Article
- Nanopore sequencing technology, bioinformatics and applications.Nature biotechnology · 2021Review
- Forensic nanopore sequencing of STRs and SNPs using Verogen's ForenSeq DNA Signature Prep Kit and MinION.International journal of legal medicine · 2021Article
- Evaluating the lettuce metatranscriptome with MinION sequencing for future spaceflight food production applications.NPJ microgravity · 2021Article
- Chromosome-level genome assemblies of the malaria vectors Anopheles coluzzii and Anopheles arabiensis.GigaScience · 2021Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The future of infectious disease surveillance and outbreak response is trending towards smaller hand-held solutions for point-of-need pathogen detection. Here, samples of Culex cedecei mosquitoes collected in Southern Florida, USA were tested for Venezuelan Equine Encephalitis Virus (VEEV), a previously-weaponized arthropod-borne RNA-virus capable of causing acute and fatal encephalitis in animal and human hosts. A single 20-mosquito pool tested positive for VEEV by quantitative reverse transcription polymerase chain reaction (RT-qPCR) on the Biomeme two3. The virus-positive sample was subjected to unbiased metatranscriptome sequencing on the Oxford Nanopore MinION and shown to contain Everglades Virus (EVEV), an alphavirus in the VEEV serocomplex. Our results demonstrate, for the first time, the use of unbiased sequence-based detection and subtyping of a high-consequence biothreat pathogen directly from an environmental sample using field-forward protocols. The development and validation of methods designed for field-based diagnostic metagenomics and pathogen discovery, such as those suitable for use in mobile "pocket laboratories", will address a growing demand for public health teams to carry out their mission where it is most urgent: at the point-of-need.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.