ArticleScientific reports2018
Low affinity uniporter carrier proteins can increase net substrate uptake rate by reducing efflux.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
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- Quantifying intracellular glucose levels when yeast is grown in glucose media.Scientific reports · 2023Article
- A New Mechanism of Carbon Metabolism and Acetic Acid Balance Regulated by CcpA.Microorganisms · 2023Article
- Structure and mechanism of oxalate transporter OxlT in an oxalate-degrading bacterium in the gut microbiota.Nature communications · 2023Article
- Mechanistic aspects of IPTG (isopropylthio-β-galactoside) transport across the cytoplasmic membrane of Escherichia coli-a rate limiting step in the induction of recombinant protein expression.Journal of industrial microbiology & biotechnology · 2023Article
- Perfect adaptation achieved by transport limitations governs the inorganic phosphate response inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Using Kinetic Modelling to Infer Adaptations inMetabolites · 2023Article
- Improving recombinant protein production by yeast through genome-scale modeling using proteome constraints.Nature communications · 2022Article
- Influence of the Zeolite ZSM-22 Precursor on a UF-PES Selective Substrate Layer for Salts Rejection.Membranes · 2022Article
- Functional characterization of Atlantic salmon (Salmo salar L.) PepT2 transporters.The Journal of physiology · 2022Article
- Multiple nutrient transporters enable cells to mitigate a rate-affinity tradeoff.PLoS computational biology · 2022Article
- Monitoring Intracellular Metabolite Dynamics inMetabolites · 2022Article
- Development and quantitative analysis of a biosensor based on the Arabidopsis SWEET1 sugar transporter.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Machine Learning Prediction of Resistance to Subinhibitory Antimicrobial Concentrations from Escherichia coli Genomes.mSystems · 2021Article
- Molecular Insight into the Regulation of Vimentin by Cysteine Modifications and Zinc Binding.Antioxidants (Basel, Switzerland) · 2021Article
- Mechanistic model of nutrient uptake explains dichotomy between marine oligotrophic and copiotrophic bacteria.PLoS computational biology · 2021Article
- Novel insights into the organic solute transporter alpha/beta, OSTα/β: From the bench to the bedside.Pharmacology & therapeutics · 2020Review
- Minimal exposure of lipid II cycle intermediates triggers cell wall antibiotic resistance.Nature communications · 2019Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many organisms have several similar transporters with different affinities for the same substrate. Typically, high-affinity transporters are expressed when substrate is scarce and low-affinity ones when it is abundant. The benefit of using low instead of high-affinity transporters remains unclear, especially when additional nutrient sensors are present. Here, we investigate two hypotheses. It was previously hypothesized that there is a trade-off between the affinity and the catalytic efficiency of transporters, and we find some but no definitive support for it. Additionally, we propose that for uptake by facilitated diffusion, at saturating substrate concentrations, lowering the affinity enhances the net uptake rate by reducing substrate efflux. As a consequence, there exists an optimal, external-substrate-concentration dependent transporter affinity. A computational model of Saccharomyces cerevisiae glycolysis shows that using the low affinity HXT3 transporter instead of the high affinity HXT6 enhances the steady-state flux by 36%. We tried to test this hypothesis with yeast strains expressing a single glucose transporter modified to have either a high or a low affinity. However, due to the intimate link between glucose perception and metabolism, direct experimental proof for this hypothesis remained inconclusive. Still, our theoretical results provide a novel reason for the presence of low-affinity transport systems.
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