ArticleVeterinary microbiology2018
Evaluating the most appropriate pooling ratio for EDTA blood samples to detect Bluetongue virus using real-time RT-PCR.
Article in Veterinary microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Preparation for a potential outbreak of bluetongue virus in Ireland: surveillance design to estimate local prevalence after an initial case detection.Irish veterinary journal · 2025Article
- Evaluation of Mosquito Blood Meals as a Tool for Wildlife Pathogen Surveillance.Pathogens (Basel, Switzerland) · 2025Article
- Saving Resources: SARS-CoV-2 Diagnostics by Real-Time RT-PCR Using Reduced Reaction Volumes.Diseases (Basel, Switzerland) · 2021Article
- Article
- Risk-based surveillance for bluetongue virus in cattle on the south coast of England in 2017 and 2018.The Veterinary record · 2020Article
- Simultaneous Detection of Bluetongue Virus Serotypes Using xMAP Technology.Microorganisms · 2020Article
- Article
- BTV-14 Infection in Sheep Elicits Viraemia with Mild Clinical Symptoms.Microorganisms · 2020Article
- Evidence of reduced viremia, pathogenicity and vector competence in a re-emerging European strain of bluetongue virus serotype 8 in sheep.Transboundary and emerging diseases · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The control of Bluetongue virus (BTV) presents a significant challenge to European Union (EU) member states as trade restrictions are placed on animals imported from BTV-affected countries. BTV surveillance programs are costly to maintain, thus, pooling of EDTA blood samples is used to reduce costs and increase throughput. We investigated different pooling ratios (1:2, 1:5, 1:10 and 1:20) for EDTA blood samples to detect a single BTV positive animal. A published real-time RT-PCR assay (Hofmann et al., 2008) and a commercial assay (ThermoFisher VetMax™ BTV NS3 kit) were used to analyse BTV RNA extracted from pooled EDTA blood samples. The detection rate was low for the onset of infection sample (0-2 days post infection (dpi); C
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Registered trials
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