Evidence map›Paper›PMID 29614952›Full record

ArticleBMC microbiology2018

Mutations of DnaA-boxes in the oriR region increase replication frequency of the MiniR1-1 plasmid.

Yuan Yao, Sukhbold Enkhtsetseg, Ingvild Odsbu, Lifei Fan, Morigen Morigen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Yuan YaoState Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock,School of Life Sciences, Inner Mongolia University, Hohhot, 010070, China.
Sukhbold EnkhtsetsegState Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock,School of Life Sciences, Inner Mongolia University, Hohhot, 010070, China.
Ingvild OdsbuDepartment of Public Health Sciences, Karolinska Institutet, Stockholm, Sweden.
Lifei FanState Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock,School of Life Sciences, Inner Mongolia University, Hohhot, 010070, China. lifei.fan@imu.edu.cn.
Morigen MorigenState Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock,School of Life Sciences, Inner Mongolia University, Hohhot, 010070, China. morigenm@hotmail.com.ORCID 0000-0001-5782-376X
Inner Mongolia University · CNKarolinska Institutet · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe MiniR1-1 plasmid is a derivative of the R1 plasmid, a low copy cloning vector.

resultsNucleotide sequencing analysis shows that the MiniR1-1 plasmid is a 6316 bp circular double-stranded DNA molecule with an oriR1 (origin for replication). The plasmid carries the repA, tap, copA and bla genes, and genes for ORF1 and ORF2. MiniR1-1 contains eight DnaA-binding sites (DnaA-boxes). DnaA-box1 is in the oriR1 region and fully matched to the DnaA-box consensus sequence, and DnaA-box8, with one mismatch, is close to the copA gene. The presence of the MiniR1-1 plasmid leads to an accumulation of the D-period cells and an increase in cell size of slowly growing Escherichia coli cells, suggesting that the presence of MiniR1-1 delays cell division. Mutations in the MiniR1-1 DnaA-box1 and DnaA-box8 significantly increase the copy number of the plasmid and the mutations in DnaA-box1 also affect cell size. It is likely that titration of DnaA to DnaA-boxes negatively controls replication of the MiniR1-1 plasmid and delays cell division. Interestingly, DnaA weakly interacts with the initiator protein RepA in vivo.

conclusionDnaA regulates the copy number of MiniR1-1 as a negative factor through interacting with the RepA protein.

Indexed as

DNA ReplicationMutationBacterial ProteinsBase SequenceBinding SitesCopper-Transporting ATPasesDNADNA, BacterialDNA-Binding ProteinsDNA HelicasesEscherichia coliEscherichia coli ProteinsGenetic VectorsPlasmidsReplication OriginSequence Analysis, DNABacterial ProteinscopA protein, E coliCopper-Transporting ATPasesDNADnaA protein, BacteriaDNA, BacterialDNA-Binding ProteinsDNA HelicasesEscherichia coli Proteinsreplication initiator proteinTrans-ActivatorsComplete genome sequenceDnaA-boxesE. coli Cell cycleMiniR1–1 replication

Identifiers

PMID29614952
PMCPMC5883639
OpenAlexW2806965465

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.