Evidence map›Paper›PMID 29614044›Full record

ArticleToxins2018

Toxic Cyanobacteria in Svalbard: Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method.

Julia Kleinteich, Jonathan Puddick, Susanna A Wood, Falk Hildebrand, H Dail Laughinghouse, David A Pearce, Daniel R Dietrich, Annick Wilmotte

Open access · goldAbstract read
In one paragraph

Article in Toxins, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
3.6field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 39 citations in OpenAlex.

  1. Article
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  4. Review
  5. Article
  6. Article
  7. Proteome-Based Serotyping of the Food-Borne PathogensMolecules (Basel, Switzerland) · 2022
    Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Review
  16. Novel Microcystins fromMarine drugs · 2019
    Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 6 institutions in 5 countries.

Julia KleinteichCenter for Applied Geosciences, Eberhard Karls Universität Tübingen, Hölderlinstr. 12, 72074 Tübingen, Germany. Julia.Kleinteich@gmail.com.
Jonathan PuddickCawthron Institute, Halifax Street East, Nelson 7010, New Zealand. awilmotte@uliege.be.ORCID 0000-0001-7000-9180
Susanna A WoodCawthron Institute, Halifax Street East, Nelson 7010, New Zealand. susie.wood@cawthron.org.nz.ORCID 0000-0003-1976-8266
Falk HildebrandStructural and Computational Biology, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany. falk.hildebrand@gmail.com.ORCID 0000-0002-0078-8948
H Dail LaughinghouseFort Lauderdale Research and Education Center, University of Florida, Davie, FL 33314, USA. hlaughinghouse@ufl.edu.ORCID 0000-0003-1018-6948
David A PearceDepartment of Applied Sciences, Faculty of Health and Life Sciences, University of Northumbria at Newcastle, Newcastle NE1 8ST, UK. david.pearce@northumbria.ac.uk.
Daniel R DietrichHuman and Environmental Toxicology, University of Konstanz, 78457 Konstanz, Germany. daniel.dietrich@uni-konstanz.de.
Annick WilmotteBCCM/ULC, University of Liege, In-Bios Centre for Protein Engineering, B6, 4000 Liege, Belgium. jonathan.puddick@cawthron.org.nz.ORCID 0000-0001-7000-9180
Cawthron Institute · NZUniversity of Liège · BEBritish Antarctic Survey · GBEuropean Molecular Biology Laboratory · DEUniversity of Florida · USUniversity of Konstanz · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cyanobacteria synthesize a large variety of secondary metabolites including toxins. Microcystins (MCs) with hepato- and neurotoxic potential are well studied in bloom-forming planktonic species of temperate and tropical regions. Cyanobacterial biofilms thriving in the polar regions have recently emerged as a rich source for cyanobacterial secondary metabolites including previously undescribed congeners of microcystin. However, detection and detailed identification of these compounds is difficult due to unusual sample matrices and structural congeners produced. We here report a time-efficient liquid chromatography-mass spectrometry (LC-MS) precursor ion screening method that facilitates microcystin detection and identification. We applied this method to detect six different MC congeners in 8 out of 26 microbial mat samples of the Svalbard Archipelago in the Arctic. The congeners, of which [Asp³, ADMAdda⁵, Dhb⁷] MC-LR was most abundant, were similar to those reported in other polar habitats. Microcystins were also determined using an Adda-specific enzyme-linked immunosorbent assay (Adda-ELISA).

Indexed as

Chromatography, LiquidCyanobacteriaEnvironmental MonitoringMass SpectrometryMicrocystinsPhylogenyRNA, Ribosomal, 16SSvalbardWater PollutantsMicrocystinsRNA, Ribosomal, 16SWater Pollutants16S rRNA genearcticbenthic matscyanotoxinsELISA

Identifiers

PMID29614044
PMCPMC5923313
OpenAlexW2796385167

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.