ArticleAMB Express2018
Cloning and expression of a novel α-1,3-arabinofuranosidase from Penicillium oxalicum sp. 68.
Article in AMB Express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed, 17 citations in OpenAlex.
- Recent advances in enzymatic properties, preparation methods, and functions of glycoside hydrolase from Bifidobacterium: a review.World journal of microbiology & biotechnology · 2023Review
- Cloning, Expression, Purification and Characterization of the β-galactosidase PoβGal35A from Penicillium oxalicum.Molecular biotechnology · 2023Article
- Molecular Cloning and Characterization of a Novel Exo-β-1,3-Galactanase fromJournal of microbiology and biotechnology · 2022Article
- A Glycoside Hydrolase Family 62 A-L-Arabinofuranosidase fromFoods (Basel, Switzerland) · 2020Article
- Screening of a Novel Polysaccharide Lyase Family 10 Pectate Lyase fromMolecules (Basel, Switzerland) · 2018Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The discovery and creation of biocatalysts for plant biomass conversion are essential for industrial demand and scientific research of the plant cell wall. α-1,2 and α-1,3-L-arabinofuranosidases are debranching enzymes that catalyzing hydrolytic release of α-L-arabinofuranosyl residues in plant cell wall. Gene database analyses shows that GH62 family only contains specific α-L-arabinofuranosidases that play an important role in the degradation and structure of the plant cell wall. At present, there are only 22 enzymes in this group has been characterized. In this study, we cloned a novel α-1,3-arabinofuranosidase gene (poabf62a) belonging to glycoside hydrolase family 62 from Penicillium oxalicum sp. 68 and expressed it in Pichia pastoris. The molecular mass of recombinant PoAbf62A was estimated to be 32.9 kDa. Using p-nitrophenyl-α-l-arabinofuranoside (pNPαAbf) as substrate, purified PoAbf62A exhibited an optimal pH of 4.5 and temperature of 35 °C. Results of methylation and
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Registered trials
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