ArticleInvestigative ophthalmology & visual science2018
Mast Cells Initiate the Recruitment of Neutrophils Following Ocular Surface Injury.
Article in Investigative ophthalmology & visual science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 44 citations in OpenAlex.
- Acute Inflammation Drives Corneal Pathological Regeneration.Investigative ophthalmology & visual science · 2026Article
- Advances in the Medical Management of Pediatric Blepharokeratoconjunctivitis.Advances in therapy · 2026Review
- Macrophages Transdifferentiate Into Myofibroblasts During Ocular Surface Inflammation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Shared Phenotypes of Immune Cells Recruited to the Cornea and the Surface of the Lens in Response to Formation of Corneal Erosions.The American journal of pathology · 2025Article
- Tissue Inhibitor of Metalloproteinase 2 Promotes Wound Healing by Suppressing Matrix Metalloproteinases and Inflammatory Cytokines in Corneal Epithelial Cells.The American journal of pathology · 2025Article
- Constructing a disease-specific ceRNA coregulatory network for keratoconus diagnosis and landscape of the immune environment.International ophthalmology · 2025Article
- Between good and evil: Complexation of the human cathelicidin LL-37 with nucleic acids.Biophysical journal · 2024Review
- Interleukin-11 Suppresses Ocular Surface Inflammation and Accelerates Wound Healing.Investigative ophthalmology & visual science · 2023Article
- Metabolomics for identifying pathways involved in vesicating agent lewisite-induced corneal injury.Experimental eye research · 2023Article
- GPR35 and mediators from platelets and mast cells in neutrophil migration and inflammation.Immunological reviews · 2023Review
- Increased activity of lacrimal gland mast cells are associated with corneal epitheliopathy in aged mice.npj aging · 2023Article
- A metabolic perspective of the neutrophil life cycle: new avenues in immunometabolism.Frontiers in immunology · 2023Review
- Corneal stromal repair and regeneration.Progress in retinal and eye research · 2022Review
- Non-immune and immune functions of interleukin-36γ suppress epithelial repair at the ocular surface.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Ocular surface mast cells promote inflammatory lymphangiogenesis.Microvascular research · 2022Article
- TRPV1Mucosal immunology · 2022Article
- Immune regulation of the ocular surface.Experimental eye research · 2022Review
- Role of Damage-Associated Molecular Patterns (DAMPs/Alarmins) in Severe Ocular Allergic Diseases.Cells · 2022Review
- Characterization of Recruited Mononuclear Phagocytes following Corneal Chemical Injury.International journal of molecular sciences · 2022Article
- Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
Abstract
Purpose: The purpose of this study was to investigate the contribution of mast cells to early neutrophil recruitment during ocular inflammation. Methods: In a murine model of corneal injury, the epithelium and anterior stroma were removed using a handheld motor brush. Cromolyn sodium (2% in PBS) eye drops were administered topically for mast cell inhibition. In vitro, bone marrow-derived mast cells were cultured alone or with corneal tissue. The frequencies of CD45+ inflammatory cells, CD11b+Ly6G+ neutrophils, and ckit+FcεR1+ mast cells in the cornea were assessed by flow cytometry. mRNA expression of CXCL2 was evaluated by real-time PCR and protein expression by ELISA. β-Hexosaminidase assays were performed to gauge mast cell activation. Results: Neutrophil infiltration of the cornea was observed within 1 hour of injury, with neutrophil frequencies increasing over subsequent hours. Concurrent expansion of mast cell frequencies at the cornea were observed, with mast cell activation (assessed by β-hexosaminidase levels) peaking at 6 hours after injury. Evaluation of CXCL2 mRNA and protein expression levels demonstrated augmented expression by injured corneal tissue relative to naïve corneal tissue. Mast cells were observed to constitutively express CXCL2, with significantly higher expression of CXCL2 protein compared with naïve corneal tissue. Culture with harvested injured corneas further amplified CXCL2 expression by mast cells. In vivo, mast cell inhibition was observed to decrease CXCL2 expression, limit early neutrophil infiltration, and reduce inflammatory cytokine expression by the cornea. Conclusions: Our data suggest that mast cell activation after corneal injury amplifies their secretion of CXCL2 and promotes the initiation of early neutrophil recruitment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.