ArticleProceedings of the National Academy of Sciences of the United States of America2018
Label-free and charge-sensitive dynamic imaging of lipid membrane hydration on millisecond time scales.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Second harmonic imaging of membrane potentials: Comparing water and chromophores as probes.Biophysical journal · 2026Article
- Interleaflet Translocation of Second-Harmonic-Generation-Active Dye Molecules in Phospholipid Bilayers with Transmembrane Pores.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Dynamic Second Harmonic Imaging of Proton Translocation Through Water Needles in Lipid Membranes.Journal of the American Chemical Society · 2024Article
- A New Theory about Interfacial Proton Diffusion Revisited: The Commonly Accepted Laws of Electrostatics and Diffusion Prevail.Biomolecules · 2023Article
- Passive transport of CaBiophysical journal · 2023Article
- Ion-Induced Transient Potential Fluctuations Facilitate Pore Formation and Cation Transport through Lipid Membranes.Journal of the American Chemical Society · 2022Article
- Monitoring membranes: The exploration of biological bilayers with second harmonic generation.Chemical physics reviews · 2022Review
- Lipid Melting Transitions Involve Structural Redistribution of Interfacial Water.The journal of physical chemistry. B · 2021Article
- Anomalous interfacial dynamics of single proton charges in binary aqueous solutions.Science advances · 2021Article
- Optical Electrophysiology: Toward the Goal of Label-Free Voltage Imaging.Journal of the American Chemical Society · 2021Article
- Water at charged interfaces.Nature reviews. Chemistry · 2021Review
- Handy water: Chiral superstructures around peptide β-sheets.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The ecological roles of microbial lipopeptides: Where are we going?Computational and structural biotechnology journal · 2021Review
- Out-of-Plane Nanoscale Reorganization of Lipid Molecules and Nanoparticles Revealed by Plasmonic Spectroscopy.The journal of physical chemistry letters · 2020Article
- Transient domains of ordered water induced by divalent ions lead to lipid membrane curvature fluctuations.Communications chemistry · 2020Article
- Label-free Optical Imaging of Membrane Potential.Current opinion in biomedical engineering · 2019Article
- Membrane water for probing neuronal membrane potentials and ionic fluxes at the single cell level.Nature communications · 2018Article
- Single-file transport of water through membrane channels.Faraday discussions · 2018Article
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4 authors.
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Abstract
Biological membranes are highly dynamic and complex lipid bilayers, responsible for the fate of living cells. To achieve this function, the hydrating environment is crucial. However, membrane imaging typically neglects water, focusing on the insertion of probes, resonant responses of lipids, or the hydrophobic core. Owing to a recent improvement of second-harmonic (SH) imaging throughput by three orders of magnitude, we show here that we can use SH microscopy to follow membrane hydration of freestanding lipid bilayers on millisecond time scales. Instead of using the UV/VIS resonant response of specific membrane-inserted fluorophores to record static SH images over time scales of >1,000 s, we SH imaged symmetric and asymmetric lipid membranes, while varying the ionic strength and pH of the adjacent solutions. We show that the nonresonant SH response of water molecules aligned by charge-dipole interactions with charged lipids can be used as a label-free probe of membrane structure and dynamics. Lipid domain diffusion is imaged label-free by means of the hydration of charged domains. The orientational ordering of water is used to construct electrostatic membrane potential maps. The average membrane potential depends quadratically on an applied external bias, which is modeled by nonlinear optical theory. Spatiotemporal fluctuations on the order of 100-mV changes in the membrane potential are seen. These changes imply that membranes are very dynamic, not only in their structure but also in their membrane potential landscape. This may have important consequences for membrane function, mechanical stability, and protein/pore distributions.
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