ArticleMicrobiome2018
The active microbial community more accurately reflects the anaerobic digestion process: 16S rRNA (gene) sequencing as a predictive tool.
Article in Microbiome, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 6 citations in OpenAlex.
- Third-generation RNA Amplicon Sequencing Reveals the Dynamics of Microbial Communities in the Tidal Reach of a Subtropical Estuary.Microbial ecology · 2026Article
- Selective survival of anaerobic digester microbes afterWater science and technology : a journal of the International Association on Water Pollution Research · 2026Article
- Examining the Effects: Lack of Impact by Endolysin and Phage Treatment on Rotifer and Larvae Microbiota.Antibiotics (Basel, Switzerland) · 2026Article
- Phylum-wide propionate degradation and its potential connection to poly-gamma-glutamate biosynthesis in Candidatus Cloacimonadota phylum.The ISME journal · 2026Article
- Time-series RNA metabarcoding of the activemSystems · 2025Article
- DNA stable isotope probing reveals hidden microbial carbon processing pathways in agricultural soils.Applied and environmental microbiology · 2025Article
- Combined DNA/RNA Amplicon Sequencing and Metatranscriptomics Reveals Microbial-Driven Nutrient Transformations and Core Taxa in Agriculturally Impacted Sediments.Environmental microbiology reports · 2025Article
- Time-series metatranscriptomics reveals differential salinity effects on the methanogenic food web in paddy soil.mSystems · 2025Article
- Microbiota and methanogenic activities in an anaerobic internal circulation reactor: insights into biogas production from brewery wastewater.Biodegradation · 2025Article
- Rhizosphere microbiomes of field-grownFrontiers in microbiology · 2025Article
- Individual methanogenic granules are whole-ecosystem replicates with reproducible responses to environmental cues.Environmental microbiome · 2024Article
- Revealing the Viable Microbial Community of Biofilm in a Sewage Treatment System Using Propidium Monoazide Combined with Real-Time PCR and Metagenomics.Microorganisms · 2024Article
- The isolation and identification of Bacillus velezensis ZN-S10 from vanilla (V. planifolia), and the microbial distribution after the curing process.Scientific reports · 2024Article
- The interplay between alterations in esophageal microbiota associated with Th17 immune response and impaired LC20 phosphorylation in achalasia.Journal of gastroenterology · 2024Article
- First evidence for temperature's influence on the enrichment, assembly, and activity of polyhydroxyalkanoate-synthesizing mixed microbial communities.Frontiers in systems biology · 2024Article
- The Role of Gut Microbiota and Associated Compounds in Cardiovascular Health and its Therapeutic Implications.Cardiovascular & hematological agents in medicinal chemistry · 2024Review
- Functional conservation of microbial communities determines composition predictability in anaerobic digestion.The ISME journal · 2023Article
- Inter-kingdom interactions and stability of methanogens revealed by machine-learning guided multi-omics analysis of industrial-scale biogas plants.The ISME journal · 2023Article
- Impact of phosphorus limitation on medium-chain-length polyhydroxyalkanoate production by activated sludge.Applied microbiology and biotechnology · 2023Article
- Ecology of food waste chain-elongating microbiome.Frontiers in bioengineering and biotechnology · 2023Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundAmplicon sequencing methods targeting the 16S rRNA gene have been used extensively to investigate microbial community composition and dynamics in anaerobic digestion. These methods successfully characterize amplicons but do not distinguish micro-organisms that are actually responsible for the process. In this research, the archaeal and bacterial community of 48 full-scale anaerobic digestion plants were evaluated on DNA (total community) and RNA (active community) level via 16S rRNA (gene) amplicon sequencing.
resultsA significantly higher diversity on DNA compared with the RNA level was observed for archaea, but not for bacteria. Beta diversity analysis showed a significant difference in community composition between the DNA and RNA of both bacteria and archaea. This related with 25.5 and 42.3% of total OTUs for bacteria and archaea, respectively, that showed a significant difference in their DNA and RNA profiles. Similar operational parameters affected the bacterial and archaeal community, yet the differentiating effect between DNA and RNA was much stronger for archaea. Co-occurrence networks and functional prediction profiling confirmed the clear differentiation between DNA and RNA profiles.
conclusionsIn conclusion, a clear difference in active (RNA) and total (DNA) community profiles was observed, implying the need for a combined approach to estimate community stability in anaerobic digestion.
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