ArticleEpigenetics & chromatin2018
Genome-wide analysis of gene regulation mechanisms during Drosophila spermatogenesis.
Article in Epigenetics & chromatin, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Machine learning analysis of Drosophila testis transcriptomic data reveals potential regulatory sequences.BioData mining · 2026Article
- Paralog-Dependent Specialization of Paf1C Subunit, Ctr9, for Sex Chromosome Gene Regulation and Male Germline Differentiation in Drosophila.Genes to cells : devoted to molecular & cellular mechanisms · 2025Article
- Dissecting the enhancer gene regulatory network in early Drosophila spermatogenesis.Nature communications · 2025Article
- Reduction in Gonad Development and Sperm Motility in Male Brown PlanthopperInternational journal of molecular sciences · 2025Article
- A germline PAF1 paralog complex ensures cell type-specific gene expression.Genes & development · 2024Article
- Deficiency ofInternational journal of molecular sciences · 2024Article
- Article
- Chromosome-specific maturation of the epigenome in the Drosophila male germline.bioRxiv : the preprint server for biology · 2023Article
- Comparison of genome architecture at two stages of male germline cell differentiation in Drosophila.Nucleic acids research · 2022Article
- The conserved molting/circadian rhythm regulator NHR-23/NR1F1 serves as an essential co-regulator ofDevelopment (Cambridge, England) · 2020Article
- Getting started: altering promoter choice as a mechanism for cell type differentiation.Genes & development · 2020Article
- Progress in Research on Sperm DNA Fragmentation.Medical science monitor : international medical journal of experimental and clinical research · 2020Review
- Islands of retroelements are major components of Drosophila centromeres.PLoS biology · 2019Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundDuring Drosophila spermatogenesis, testis-specific meiotic arrest complex (tMAC) and testis-specific TBP-associated factors (tTAF) contribute to activation of hundreds of genes required for meiosis and spermiogenesis. Intriguingly, tMAC is paralogous to the broadly expressed complex Myb-MuvB (MMB)/dREAM and Mip40 protein is shared by both complexes. tMAC acts as a gene activator in spermatocytes, while MMB/dREAM was shown to repress gene activity in many cell types.
resultsOur study addresses the intricate interplay between tMAC, tTAF, and MMB/dREAM during spermatogenesis. We used cell type-specific DamID to build the DNA-binding profiles of Cookie monster (tMAC), Cannonball (tTAF), and Mip40 (MMB/dREAM and tMAC) proteins in male germline cells. Incorporating the whole transcriptome analysis, we characterized the regulatory effects of these proteins and identified their gene targets. This analysis revealed that tTAFs complex is involved in activation of achi, vis, and topi meiosis arrest genes, implying that tTAFs may indirectly contribute to the regulation of Achi, Vis, and Topi targets. To understand the relationship between tMAC and MMB/dREAM, we performed Mip40 DamID in tTAF- and tMAC-deficient mutants demonstrating meiosis arrest phenotype. DamID profiles of Mip40 were highly dynamic across the stages of spermatogenesis and demonstrated a strong dependence on tMAC in spermatocytes. Integrative analysis of our data indicated that MMB/dREAM represses genes that are not expressed in spermatogenesis, whereas tMAC recruits Mip40 for subsequent gene activation in spermatocytes.
conclusionsDiscovered interdependencies allow to formulate a renewed model for tMAC and tTAFs action in Drosophila spermatogenesis demonstrating how tissue-specific genes are regulated.
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