ArticleBMC oral health2018
Influence of iRoot SP and mineral trioxide aggregate on the activation and polarization of macrophages induced by lipopolysaccharide.
Article in BMC oral health, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.
- Endodontic sealers based on calcium silicates: a systematic review.Odontology · 2019Pooled it
- Evaluation of a novel bioceramic sealer NeoSEALER Flo on the activation and polarization of macrophage.Odontology · 2026Article
- Association of salivary mmp-8 gene polymorphism with its protein expression in oral submucous fibrosis patients.Odontology · 2025Article
- Polydatin Modulates Inflammatory Cytokine Expression in Lipoteichoic Acid-Stimulated Human Dental-Pulp Stem Cells.Journal of functional biomaterials · 2025Article
- Bioactive Materials in Vital Pulp Therapy: Promoting Dental Pulp Repair Through Inflammation Modulation.Biomolecules · 2025Review
- Host defense peptides combined with MTA extract increase the repair in dental pulp cells: in vitro and ex vivo study.Scientific reports · 2023Article
- Development of Rat Caries-Induced Pulpitis Model for Vital Pulp Therapy.Journal of dental research · 2023Article
- Bioceramics in Endodontics: Updates and Future Perspectives.Bioengineering (Basel, Switzerland) · 2023Review
- Immunomodulatory Effects of Endodontic Sealers: A Systematic Review.Dentistry journal · 2023Review
- The effect of implants loaded with stem cells from human exfoliated deciduous teeth on early osseointegration in a canine model.BMC oral health · 2022Article
- Calcium Silicate-Based Root Canal Sealers: A Narrative Review and Clinical Perspectives.Materials (Basel, Switzerland) · 2021Review
- Review
- Conservative endodontic management using a calcium silicate bioceramic sealer for delayed root fracture: A case report and review of the literature.World journal of clinical cases · 2021Article
- Cytotoxicity and Bioactivity of Mineral Trioxide Aggregate and Bioactive Endodontic Type Cements: A Systematic Review.International journal of clinical pediatric dentistryReview
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBiomaterials could affect the inflammation reaction and wound healing via the activation and polarization of macrophages. However, the influence of iRoot SP and mineral trioxide aggregate (MTA) on macrophage polarization under inflammatory conditions was not reported although these two root filling materials have been applied extensively in patients undergoing endodontic treatment. Therefore, the present study aimed to explore the mechanism how iRoot SP and MTA affect the cell behavior of RAW 264.7 macrophages when stimulated by lipopolysaccharide (LPS) in vitro.
methodsThe gene expression of three main related pro-inflammatory cytokines (IL-1β, TNF-α, IL-6) was examined by quantitative real-time polymerase chain reaction (qRT-PCR) in RAW 264.7 macrophages when stimulated by iRoot SP and MTA in the presence of LPS. The protein expression of the M1 and M2 phenotype specific markers, CD11c and CD206, was assessed by immunofluorescence and flow cytometry in RAW 264.7 macrophages.
resultsLPS promoted the expression of IL-1β, TNF-α, and IL-6 in RAW 264.7 macrophages as compared to the control group. Both iRoot SP and MTA were significantly able to enhance the expression of IL-1β, TNF-α, and IL-6 in RAW 264.7 macrophages as compared to LPS group. LPS could increase the expression of CD11c as compared to the control group while iRoot SP and MTA were able to enhance the expression of both CD11c and CD206 as compared to LPS group.
conclusionsiRoot SP and MTA could potentially promote the release of pro-inflammatory cytokines in RAW 264.7 macrophages and induce into M1/M2 phenotype when cultured with LPS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.