Evidence map›Paper›PMID 29609564›Full record

ArticleBMC oral health2018

Apical periodontitis: preliminary assessment of microbiota by 16S rRNA high throughput amplicon target sequencing.

Federico Mussano, Ilario Ferrocino, Natalija Gavrilova, Tullio Genova, Alessandro Dell'Acqua, Luca Cocolin, Stefano Carossa

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC oral health, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed, 1 pooled it
4.8field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.

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  15. Autophagy in aging-related oral diseases.Frontiers in endocrinology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Federico MussanoCIR Dental School, Department of Surgical Sciences, University of Turin, via Nizza 230, 10126, Turin, Italy. federico.mussano@unito.it.ORCID 0000-0001-5800-0749
Ilario FerrocinoDISAFA - Microbiology and Food Technology sector, University of Turin, Largo Paolo Braccini n°2, 10095, Grugliasco, TO, Italy.
Natalija GavrilovaCIR Dental School, Department of Surgical Sciences, University of Turin, via Nizza 230, 10126, Turin, Italy.
Tullio GenovaCIR Dental School, Department of Surgical Sciences, University of Turin, via Nizza 230, 10126, Turin, Italy.
Alessandro Dell'AcquaAzienda Ospedaliero-Universitaria Città della Salute, San Giovanni Battista di Torino, Turin, Italy.
Luca CocolinDISAFA - Microbiology and Food Technology sector, University of Turin, Largo Paolo Braccini n°2, 10095, Grugliasco, TO, Italy.
Stefano CarossaCIR Dental School, Department of Surgical Sciences, University of Turin, via Nizza 230, 10126, Turin, Italy.
University of Turin · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundApical periodontitis includes periapical granulomas and radicular cysts, which are histologically distinguished by the absence and the presence of an epithelial lining, respectively. The main cause of apical periodontitis is the bacterial colonization of the root canal space. This research aimed at assessing whether and how periapical granulomas and radicular cysts differ in terms of microbiota using high throughput amplicon target sequencing (HTS) techniques.

methodsThis study included 5 cases of Periapical Granulomas (PGs) and 5 cases of Radicular Cysts (RCs) selected on the base of histology out of 37 patients from January 2015 to February 2016. Complete medical history, panoramic radiograms (OPTs) and histologic records of each patient were assessed. Only lesions greater than 1 cm in diameter and developed in proximity to teeth with bad prognosis were included. The microbiota present in periapical granulomas and radicular cysts thus retrieved was finely characterized by pyrosequencing of the 16S rRNA genes.

resultsThe core of OTUs shared between periapical granulomas and radicular cysts was dominated by the presence of facultative anaerobes taxa such as: Lactococcus lactis, Propionibacterium acnes, Staphylococcus warneri, Acinetobacter johnsonii and Gemellales. L. lactis, the main OTUs of the entire datasets, was associated with periapical granuloma samples. Consistently with literature, the anaerobic taxa detected were most abundant in radicular cyst samples. Indeed, a higher abundance of presumptive predicted metabolic pathways related to Lipopolysaccharide biosynthesis was found in radicular cyst samples.

conclusionsThe present pilot study confirmed the different microbial characterization of the two main apical periodontitis types and shade light on the possible role of L. lactis in periapical granulomas.

Indexed as

AdultFemaleHigh-Throughput Nucleotide SequencingHumansMaleMicrobiotaPeriapical GranulomaPeriapical PeriodontitisPilot ProjectsRadicular CystRNA, Ribosomal, 16SYoung AdultRNA, Ribosomal, 16SApical periodontitis (AP)High throughput amplicon target sequencingMicrobiotaPeriapical granulomas (PGs)Radicular cysts (RCs)

Identifiers

PMID29609564
PMCPMC5879750
OpenAlexW2800683724

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.