Evidence map›Paper›PMID 29608965›Full record

ArticleMethods (San Diego, Calif.)2018

Peak decay analysis and biointeraction studies of immunoglobulin binding and dissociation on protein G affinity microcolumns.

Jeanethe A Anguizola, Erika L Pfaunmiller, Mitchell L Milanuk, David S Hage

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods (San Diego, Calif.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 14 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Article
  5. Article
  6. Article
  7. Review
  8. Kinetic Analysis by Affinity Chromatography.Frontiers in chemistry · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Jeanethe A AnguizolaDepartment of Chemistry, University of Panama, Panama City, Panama. Electronic address: jeanethe.anguizola@up.ac.pa.
Erika L PfaunmillerDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
Mitchell L MilanukDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
David S HageDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
University of Nebraska–Lincoln · USUniversidad de Panamá · PA

Funding

CHROMATOGRAPHIC STUDIES OF FUNCTIONAL PROTEOMICSR01DK069629 · NIDDK · UNIVERSITY OF NEBRASKA LINCOLN · PI HAGE, DAVID S · 2006 to 2017
$1.8M
NIDDK NIH HHS R01 DK069629
6 · The paper itself

Abstract

Protein G can be a valuable binding agent for antibodies and immunoglobulins in methods such as immunosensors, chromatographic-based immunoassays, and immunoaffinity chromatography. This report used the method of peak decay analysis along with frontal analysis and zonal elution studies to characterize the binding, elution and regeneration properties of affinity microcolumns that contained immobilized protein G. Frontal analysis was employed with rabbit immunoglobulin G (IgG) to characterize the binding capacity of these affinity microcolumns. Zonal elution experiments looking at the retained peaks for small injections of labeled rabbit IgG were used to optimize the column regeneration conditions. Peak decay analysis was then used to look at the effects of flow rate and elution pH on the release of several types of IgG from the protein G microcolumns. This approach made it possible to obtain detailed information on the use and behavior of such columns, as could be used in future work to optimize the capture or analysis of IgG and antibodies by such devices. The same approach and tools that were used in this report could also be adapted for work with affinity columns that make use of other supports, binding agents or targets.

Indexed as

Chromatography, AffinityAnimalsImmunoassayImmunoglobulin GKineticsProtein BindingRabbitsImmunoglobulin GAffinity microcolumnDissociation rateFrontal analysisImmunoglobulin GPeak decay methodProtein G

Identifiers

PMID29608965
PMCPMC6114083
OpenAlexW2795075759

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.