ArticlePLoS genetics2018
High-throughput interaction screens illuminate the role of c-di-AMP in cyanobacterial nighttime survival.
Article in PLoS genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 50 citations in OpenAlex.
- Effects of the signaling molecule cyclic-di-GMP on cyanobacterial circadian rhythm inJournal of bacteriology · 2026Article
- Altered abundance in cancer patients gut of diadenylate cyclase-encoding bacteria.Scientific reports · 2026Article
- Diurnal rhythm causes metabolic crises in the cyanobacterial mutants of c-di-AMP signaling cascade.iScience · 2025Article
- The second messenger c-di-AMP controls natural competence via ComFB signaling protein.Cell discovery · 2025Article
- Impact of c-di-AMP Accumulation, L-cysteine, and Oxygen on Catalase Activity and Oxidative Stress Resistance ofMicroorganisms · 2025Article
- Bacterial cell volume regulation and the importance of cyclic di-AMP.Microbiology and molecular biology reviews : MMBR · 2024Review
- All DACs in a Row: Domain Architectures of Bacterial and Archaeal Diadenylate Cyclases.Journal of bacteriology · 2023Review
- Genomic Distribution of Pro-VirulentInternational journal of molecular sciences · 2023Article
- Cyclic di-AMP, a multifaceted regulator of central metabolism and osmolyte homeostasis inmicroLife · 2023Review
- Roles of second messengers in the regulation of cyanobacterial physiology: the carbon-concentrating mechanism and beyond.microLife · 2023Review
- Transcriptomic and Phenomic Investigations Reveal Elements in Biofilm Repression and Formation in the CyanobacteriumFrontiers in microbiology · 2022Article
- Enzyme Characterization of Pro-virulent SntA, a Cell Wall-Anchored Protein ofFrontiers in microbiology · 2022Article
- Diurnal metabolic control in cyanobacteria requires perception of second messenger signaling molecule c-di-AMP by the carbon control protein SbtB.Science advances · 2021Article
- A Rationally Designed c-di-AMP Förster Resonance Energy Transfer Biosensor To Monitor Nucleotide Dynamics.Journal of bacteriology · 2021Article
- The Diadenylate Cyclase CdaA Is Critical for Borrelia turicatae Virulence and Physiology.Infection and immunity · 2021Article
- Improved photosynthetic capacity and photosystem I oxidation via heterologous metabolism engineering in cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Essentiality of c-di-AMP in Bacillus subtilis: Bypassing mutations converge in potassium and glutamate homeostasis.PLoS genetics · 2021Article
- Gene Regulation and Transcriptomics.Current issues in molecular biology · 2021Article
- The Many Roles of the Bacterial Second Messenger Cyclic di-AMP in Adapting to Stress Cues.Journal of bacteriology · 2020Review
- A decade of research on the second messenger c-di-AMP.FEMS microbiology reviews · 2020Review
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
Abstract
The broadly conserved signaling nucleotide cyclic di-adenosine monophosphate (c-di-AMP) is essential for viability in most bacteria where it has been studied. However, characterization of the cellular functions and metabolism of c-di-AMP has largely been confined to the class Bacilli, limiting our functional understanding of the molecule among diverse phyla. We identified the cyclase responsible for c-di-AMP synthesis and characterized the molecule's role in survival of darkness in the model photosynthetic cyanobacterium Synechococcus elongatus PCC 7942. In addition to the use of traditional genetic, biochemical, and proteomic approaches, we developed a high-throughput genetic interaction screen (IRB-Seq) to determine pathways where the signaling nucleotide is active. We found that in S. elongatus c-di-AMP is produced by an enzyme of the diadenylate cyclase family, CdaA, which was previously unexplored experimentally. A cdaA-null mutant experiences increased oxidative stress and death during the nighttime portion of day-night cycles, in which potassium transport is implicated. These findings suggest that c-di-AMP is biologically active in cyanobacteria and has non-canonical roles in the phylum including oxidative stress management and day-night survival. The pipeline and analysis tools for IRB-Seq developed for this study constitute a quantitative high-throughput approach for studying genetic interactions.
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Registered trials
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