ArticleACS synthetic biology2018
Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit for Modular Assembly of Broad-Host-Range Plasmids.
Article in ACS synthetic biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 143 citations in OpenAlex.
- An engineered bacterial symbiont maps micron-scale sugar gradients in the honeybee gut.PLoS biology · 2026Article
- Colibactin produced by a honeybee gut symbiont mediates pathogen defence.Nature microbiology · 2026Article
- Genetic Biosensor for Optimizing Double-Stranded RNA Production by Bacteria.ACS synthetic biology · 2026Article
- Symbiont-Mediated Detoxification of Xenobiotics in Honey Bees.Microbial biotechnology · 2026Review
- Proof-of-concept engineering of Escherichia coli expressing a bee-derived cytochrome P450 monooxygenase for thiamethoxam detoxification.Journal of biological engineering · 2026Article
- A Comparative Survey of Soldier Fly (Stratiomyidae) Larval Gut Microbiomes Across Five Subfamilies Reveals Novel Bacterial Diversity and a "Wild Core" in Hermetia illucens.Microbial ecology · 2026Article
- Refactored genetic parts for modular assembly of the E. coli MccV type I secretion system used to screen class II microcin candidates from plant-associated bacteria.BMC biotechnology · 2026Article
- Article
- Longevity and foraging performance of honey bees treated with an RNAi-based Varroa destructor biopesticide.Scientific reports · 2026Article
- The Coli Toolkit (CTK): An Extension of the Modular Yeast Toolkit for Use inACS synthetic biology · 2026Article
- Gut microbiota-derived butyrate primes systemic immunity in honey bees by mediating lipid metabolic reprogramming.Nature communications · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Expression of a phage RecET recombinase system improves detection of shorter DNA fragments by anmicroPublication biology · 2026Article
- Colibactin produced by a honeybee symbiont defends against pathogens and shapes the gut community.bioRxiv : the preprint server for biology · 2025Article
- Pathogenicity and transmission of Morganella morganii in honey bees.PLoS pathogens · 2025Article
- A-to-I mRNA editing recodes hundreds of genes in dozens of species and produces endogenous protein isoforms in bacteria.Nucleic acids research · 2025Article
- MyD88 knockdown by RNAi prevents bacterial stimulation of tubeworm metamorphosis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Variance in C. elegans gut bacterial load suggests complex host-microbe dynamics.PLoS computational biology · 2025Article
- Air pollution modifies colonisation factors in beneficial symbiont Snodgrassella and disrupts the bumblebee gut microbiome.NPJ biofilms and microbiomes · 2025Article
- Life history strategies complement niche partitioning to support the coexistence of closely related Gilliamella species in the bee gut.The ISME journal · 2025Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
Engineering the bacteria present in animal microbiomes promises to lead to breakthroughs in medicine and agriculture, but progress is hampered by a dearth of tools for genetically modifying the diverse species that comprise these communities. Here we present a toolkit of genetic parts for the modular construction of broad-host-range plasmids built around the RSF1010 replicon. Golden Gate assembly of parts in this toolkit can be used to rapidly test various antibiotic resistance markers, promoters, fluorescent reporters, and other coding sequences in newly isolated bacteria. We demonstrate the utility of this toolkit in multiple species of Proteobacteria that are native to the gut microbiomes of honey bees ( Apis mellifera) and bumble bees (B ombus sp.). Expressing fluorescent proteins in Snodgrassella alvi, Gilliamella apicola, Bartonella apis, and Serratia strains enables us to visualize how these bacteria colonize the bee gut. We also demonstrate CRISPRi repression in B. apis and use Cas9-facilitated knockout of an S. alvi adhesion gene to show that it is important for colonization of the gut. Beyond characterizing how the gut microbiome influences the health of these prominent pollinators, this bee microbiome toolkit (BTK) will be useful for engineering bacteria found in other natural microbial communities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.