ArticleJournal of visualized experiments : JoVE2018
A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed, 60 citations in OpenAlex.
- Acute and lifelong exercise modulate the tumorigenic potential of human lung cancer cells and their susceptibility to cisplatin.Experimental physiology · 2026Article
- In vitro and in vivo evaluation of oleuropein loaded hyalurosomes for diabetic foot ulcer healing.Scientific reports · 2026Article
- Engineered mesenchymal stem cells expressing CXCR4 and LIGHT enhance Anti-Tumor activity in a subcutaneous gastric cancer xenograft model.Medical oncology (Northwood, London, England) · 2026Article
- In vitro wound simulation: A high-throughput device for scratch assays.Methods (San Diego, Calif.) · 2026Article
- Bioactive derivatives of the antimicrobial peptide esculentin-1a promote human dermal fibroblast migration and activate genes involved in early wound healing.BBA advances · 2026Article
- Ethanolic extract of Otostegia fruticosa induces ROS-dependent apoptosis and reduces migration of MDA-MB-231 cells in vitro.PloS one · 2026Article
- Selecting the optimal cell migration assay: fundamentals and practical guidelines.Nature methods · 2026Review
- Microfluidics, 3D bioprinted scaffold and organ-on-chip with biosensing activated therapeutic strategies for diabetic wound healing.Frontiers in bioengineering and biotechnology · 2026Review
- A novel method for evaluating and visualizing scratch wound healing assays using level-set and image sector analysis.PNAS nexus · 2025Article
- In Vitro Wound Simulation: A High-Throughput Device for Scratch Assays.bioRxiv : the preprint server for biology · 2025Article
- Anti-Inflammatory and Antimicrobial Effect of Ellagic Acid and Punicalagin in Dermal Fibroblasts.International journal of molecular sciences · 2025Article
- Anticancer and Anti-Inflammatory Effects of Benzothiazole Derivatives Targeting NF-κB/COX-2/iNOS in a Hepatocellular Carcinoma Cell Line.Turkish journal of pharmaceutical sciences · 2025Article
- In Vitro and In Silico Wound-Healing Activity of Two Cationic Peptides Derived from Cecropin D inAntibiotics (Basel, Switzerland) · 2025Article
- Wound Healing, Antioxidant, and Antiviral Properties of Bioactive Polysaccharides of Microalgae Strains Isolated from Greek Coastal Lagoons.Marine drugs · 2025Article
- Evaluation of extracts from Phyllostachys makinoi for their antibacterial and accelerated wound healing potential.AMB Express · 2025Article
- GFAPβ and GFAPδ Isoforms Expression in Mesenchymal Stem Cells, MSCs Differentiated Towards Schwann-like, and Olfactory Ensheathing Cells.Current issues in molecular biology · 2025Article
- Article
- Ethanolic Extract ofPharmaceutics · 2024Article
- A programmable platform for probing cell migration and proliferation.APL bioengineering · 2024Article
- A high-precision wound healing assay based on photosensitized culture substrates.Scientific reports · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this work is to show a novel method to evaluate the ability of some immunomodulatory molecules, such as antimicrobial peptides (AMPs), to stimulate cell migration. Importantly, cell migration is a rate-limiting event during the wound-healing process to re-establish the integrity and normal function of tissue layers after injury. The advantage of this method over the classical assay, which is based on a manually made scratch in a cell monolayer, is the usage of special silicone culture inserts providing two compartments to create a cell-free pseudo-wound field with a well-defined width (500 μm). In addition, due to an automated image analysis platform, it is possible to rapidly obtain quantitative data on the speed of wound closure and cell migration. More precisely, the effect of two frog-skin AMPs on the migration of bronchial epithelial cells will be shown. Furthermore, pretreatment of these cells with specific inhibitors will provide information on the molecular mechanisms underlying such events.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.