ArticleJournal of visualized experiments : JoVE2018
Isolation of Dendritic Cells from the Human Female Reproductive Tract for Phenotypical and Functional Studies.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed.
- The immune milieu after local endometrial injury in women with recurrent implantation failure.Scientific reports · 2025Article
- Decoding functional and developmental trajectories of tissue-resident uterine dendritic cells through integrative omics.iScience · 2025Article
- Inflammatory, Functional, and Compositional Changes of the Uterine Immune Microenvironment in a Lymphangioleiomyomatosis Mouse Model.Journal of cellular immunology · 2025Article
- Human genital dendritic cell heterogeneity confers differential rapid response to HIV-1 exposure.Frontiers in immunology · 2024Article
- Impact of aging on the frequency, phenotype, and function of CD4+ T cells in the human female reproductive tract.Frontiers in immunology · 2024Article
- Aging modifies endometrial dendritic cell function and unconventional double negative T cells in the human genital mucosa.Immunity & ageing : I & A · 2023Article
- Aging dysregulates neutrophil extracellular trap formation in response to HIV in blood and genital tissues.Frontiers in immunology · 2023Article
- Aging beyond menopause selectively decreases CD8+ T cell numbers but enhances cytotoxic activity in the human endometrium.Immunity & ageing : I & A · 2022Article
- Direct and Indirect endocrine-mediated suppression of human endometrial CD8+T cell cytotoxicity.Scientific reports · 2021Article
- Whole-Tissue Deconvolution and scRNAseq Analysis Identify Altered Endometrial Cellular Compositions and Functionality Associated With Endometriosis.Frontiers in immunology · 2021Article
- Complement-Opsonized HIV Modulates Pathways Involved in Infection of Cervical Mucosal Tissues: A Transcriptomic and Proteomic Study.Frontiers in immunology · 2021Article
- Differential Cytotoxic Function of Resident and Non-resident CD8+ T Cells in the Human Female Reproductive Tract Before and After Menopause.Frontiers in immunology · 2020Article
- Epithelial Cells and Fibroblasts from the Human Female Reproductive Tract Accumulate and Release TFV and TAF to Sustain Inhibition of HIV Infection of CD4+ T cells.Scientific reports · 2019Article
- Human Dendritic Cell Subsets, Ontogeny, and Impact on HIV Infection.Frontiers in immunology · 2019Review
- Neutrophil extracellular traps prevent HIV infection in the female genital tract.Mucosal immunology · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The characterization of the human dendritic cells (DCs) resident in mucosal tissues is challenging due to the difficulty in obtaining samples, and the low numbers of DCs present per tissue. Yet, as the phenotype and function of DCs is modified by the tissue environment, it is necessary to analyze tissue resident DC populations, since blood derived DCs incompletely reflect the complexities of DCs in tissues. Here we present a protocol to isolate DCs from the human female reproductive tract (FRT) using hysterectomy specimens that allows both phenotypical and functional analyses. The protocol consists of tissue digestion to generate a single cell mixed cell suspension, followed by positive magnetic bead selection. Our tissue digestion protocol does not cleave surface markers, which allows phenotypical and functional analysis of DCs in the steady state, without overnight incubation or cell activation. This protocol can be adapted for the isolation of other immune cell types or isolation of DCs from other tissues.
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Registered trials
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